Study on UHPLC fingerprint and determination of eight phenolic components of Tetrastigma hemsleyanum leaves
| Autor: | Hui-Long Xu, Zhi-Sen Xie, Wen Xu, Fu-Xian Zou, Bi-Ya Cai, Xin-Yue Xie, Xin-Ping Huang, Shi-Ming Fan |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Orientin Chromatography Neochlorogenic acid Isoorientin Phytochemicals Isovitexin Vitexin computer.software_genre Plant Leaves 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Phenols Vitaceae Complementary and alternative medicine Chlorogenic acid chemistry Fingerprint Pharmacology (medical) Data mining General Pharmacology Toxicology and Pharmaceutics Quantitative analysis (chemistry) computer Chromatography High Pressure Liquid |
| Zdroj: | China Journal of Chinese Materia Medica. |
| ISSN: | 1001-5302 |
| DOI: | 10.4268/cjcmm20162115 |
| Popis: | A novel method combining ultra-high performance liquid chromatography (UHPLC) fingerprint and simultaneous quantitative analysis of eight phenolic components was developed and validated for quality evaluation of Tetrastigma hemsleyanum leaves. For fingerprint analysis, 15 peaks were selected as the common peaks to evaluate the similarities among 41 batches of T. hemsleyanum leaves collected from different regions. Additionally, simultaneous quantification of eight markers, including neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isoorientin, orientin, vitexin-2-O-rhamnoside,vitexin and isovitexin, was performed and the obtained data demonstrated that our method has achieved desired linearity, precision and accuracy. Clustering statistical analysis was further application in T. hemsleyanum leaves from different regions. The results indicated that new approach conbine ultra-high performance liquid chromatography (UHPLC) fingerprint and simultaneous quantitative analysis of eight phenolic components was applicable in quality control of T. hemsleyanum leaves. |
| Databáze: | OpenAIRE |
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