A rapid method of cryofixation of tissues in situ for ultracryomicrotomy
| Autor: | Ruth Ellen Bulger, Seung H. Chang, I. K. Berezesky, Pendergrass Re, W J Mergner, Benjamin F. Trump |
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| Rok vydání: | 1980 |
| Předmět: |
In situ
Punch Biopsy Kidney Cortex Histology Materials science Microvilli Ice crystals Histocytochemistry Scanning electron microscope Biopsy Microanalysis Basement Membrane Cryofixation Sampling device Kidney Tubules Proximal Transmission electron microscopy Freezing Humans Anatomy Biomedical engineering |
| Zdroj: | Journal of Histochemistry & Cytochemistry. 28:47-51 |
| ISSN: | 1551-5044 0022-1554 |
| DOI: | 10.1177/28.1.7351474 |
| Popis: | A device is described for the rapid freezing of tissue in situ by a punch biopsy approach using a specially designed cryogun with a highly thermal conductive specimen holder. The cryogun consists of a sampling device using a double, spring-loaded gun mechanism and a system of cryochambers. Ultrathin freeze-dried sections cut from samples obtained with this cryogun are relatively free of artifacts and have few ice crystals. Organelles are seen by natural contrast when cryosections of approximately 1000 A are observed with a transmission electron microscope or in the transmission mode of a scanning electron microscope. The construction of the cryogun is described along with a method of obtaining improved, ultrafast cryofixation of tissue specimens. The reliability of obtaining x-ray microanalysis measurements of diffusible ions where movement within cell compartments has been retained is discussed. |
| Databáze: | OpenAIRE |
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