Immunological detection of the Weligama coconut leaf wilt disease associated phytoplasma: Development and validation of a polyclonal antibody based indirect ELISA
| Autor: | Rohana Wijesekera, Preethi V. Udagama, Chamini Kanatiwela-de Silva, Malini Damayanthi, Matthew Dickinson, Nalin de Silva, Devaka Weerakoon |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Cocos Veterinary medicine Phytoplasma Science Enzyme-Linked Immunosorbent Assay Biology medicine.disease_cause 01 natural sciences Cross-reactivity law.invention 03 medical and health sciences law medicine Animals Polymerase chain reaction Areca Wilt disease Plant Diseases Multidisciplinary food and beverages biology.organism_classification Antibodies Bacterial Plant Leaves 030104 developmental biology Polyclonal antibodies Shoot biology.protein Medicine Rabbits Restriction fragment length polymorphism 010606 plant biology & botany |
| Zdroj: | PLoS ONE, Vol 14, Iss 4, p e0214983 (2019) |
| ISSN: | 1932-6203 |
| Popis: | Weligama coconut leaf wilt disease (WCLWD) causes heavy losses in the coconut cultivations of southern Sri Lanka. The in-house developed and validated indirect ELISA was based on specific polyclonal antibodies raised in female New Zealand White rabbits, against partially purified WCLWD associated phytoplasma. This ELISA has the potential to distinguish secA PCR confirmed, WCLWD associated phytoplasma positive palms from phytoplasma free palms at high accuracy (93%) and sensitivity (92.7%), but with marginal specificity (79%). The calculated ELISA cross reactivity index (CRI) values were low for sugarcane white leaf (7%) and sugarcane grassy shoot (8%) infected leaves, but with marked highCRIfor both Bermuda grass white leaf (69%) and areca nut yellow leaf (70%) infected leaves. SecA gene based phylogenetic relationships of the WCLWD associated phytoplasma with these other locally prevalent phytoplasma strains elucidated this immunological cross reactivity, which was further reiterated by virtual restriction fragment length polymorphism analysis. Based on scanning electron microscopy, this study provides additional visual evidence, for the presence of phytoplasmas in WCLWD infected tissues. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|