Impact of preconditioning with retinoic acid during early development on morphological and functional characteristics of human induced pluripotent stem cell-derived neurons
| Autor: | Jochen Utikal, Rolf-Detlef Treede, Wolfgang Greffrath, Patrick Schloss, Friederike Matthäus, Sandra Horschitz, Jan Rosner, Anja Groß, Andreas Meyer-Lindenberg, Marta Galach |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Induced Pluripotent Stem Cells
Retinoic acid Glutamic Acid Tretinoin Biology Stem cell marker chemistry.chemical_compound Directed differentiation Imaging Three-Dimensional Neural Stem Cells Humans Induced pluripotent stem cell Cell Shape lcsh:QH301-705.5 Cells Cultured Medicine(all) Neurons Gene Expression Profiling Cell Differentiation Cell Biology General Medicine Axon initial segment Axons Cell biology Up-Regulation P19 cell Phenotype Biochemistry chemistry nervous system lcsh:Biology (General) Cell culture Calcium Neural development Developmental Biology |
| Zdroj: | Stem Cell Research, Vol 15, Iss 1, Pp 30-41 (2015) |
| ISSN: | 1876-7753 1873-5061 |
| Popis: | Human induced pluripotent stem cells (hiPSCs) are a suitable tool to study basic molecular and cellular mechanisms of neurodevelopment. The directed differentiation of hiPSCs via the generation of a self-renewable neuronal precursor cell line allows the standardization of defined differentiation protocols. Here, we have investigated whether preconditioning with retinoic acid during early neural induction impacts on morphological and functional characteristics of the neuronal culture after terminal differentiation. For this purpose we have analyzed neuronal and glial cell markers, neuronal outgrowth, soma size, depolarization-induced distal shifts of the axon initial segment as well as glutamate-evoked calcium influx. Retinoic acid preconditioning led to a higher yield of neurons vs. glia cells and longer axons than unconditioned controls. In contrast, glutamatergic activation and depolarization induced structural plasticity were unchanged. Our results show that the treatment of neuroectodermal cells with retinoic acid during early development, i.e. during the neurulation phase, increases the yield of neuronal phenotypes, but does not impact on the functionality of terminally differentiated neuronal cells. |
| Databáze: | OpenAIRE |
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