Miniaturized systems for evaluating enzyme activity in polymeric membrane bioreactors
| Autor: | Mohammad Shafquatul Islam, Cindy K. Harnett |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Environmental Engineering Immobilized enzyme Bioengineering Membrane bioreactor 01 natural sciences 7. Clean energy Chemical reaction complex mixtures Catalysis laccase 03 medical and health sciences chemistry.chemical_compound 010608 biotechnology Bioreactor lignin degradation Lignin 030304 developmental biology enzyme immobilization Laccase 0303 health sciences technology industry and agriculture food and beverages Membrane chemistry Chemical engineering polymeric membrane bioreactor Biotechnology Research Article |
| Zdroj: | Engineering in Life Sciences |
| ISSN: | 1618-2863 1618-0240 |
| Popis: | Enzyme‐coated polymeric membranes are versatile catalysts for biofuel production and other chemical production from feedstock, like plant biomass. Such bioreactors are more energy efficient than high temperature methods because enzymes catalyze chemical reactions near room temperature. A major challenge in processing plant biomass is the presence of lignin, a complex aromatic polymer that resists chemical breakdown. Therefore, membranes coated with enzymes such as laccase that can degrade lignin are sought for energy extraction systems. We present an experimental study on optimizing an enzyme‐based membrane bioreactor and investigate the tradeoff between high flow rate and short dwell time in the active region. In this work, zero flow rate voltammetry experiments confirm the electrochemical activity of Trametes versicolor laccase on conductive polymer electrodes, and a flow‐through spectroscopy device with laccase‐coated porous nylon membranes is used with a colorimetric laccase activity indicator to measure the catalysis rate and percent conversion as a function of reactant flow rate. Membrane porosity before and after laccase coating is verified with electron microscopy. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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