GIT1 protects traumatically injured spinal cord by prompting microvascular endothelial cells to clear myelin debris
| Autor: | Tao Xu, Guoyong Yin, Dingfei Qian, Chenliang Zhang, Cong Li, Shujie Zhao, Jun Gu, Ming Wang, Qirui Ding, Pengyu Tang, Zheng Zhou, Qian Wang, Jian Chen, Hao Liu, Fan‐Qi Kong, Wenlin Deng, Yifan Huang, Jin Fan, Bowen Wan |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Vascular Endothelial Growth Factor A
Aging autophagy Angiogenesis myelin debris Neovascularization Physiologic Cell Cycle Proteins Myelin angiogenesis In vivo medicine Animals Receptor Spinal cord injury Cells Cultured Myelin Sheath Spinal Cord Injuries Mice Knockout Chemistry Kinase Autophagy GTPase-Activating Proteins Endothelial Cells Cell Biology Spinal cord medicine.disease spinal cord injury Cell biology Up-Regulation medicine.anatomical_structure Microvessels GIT1 Research Paper |
| Zdroj: | Aging (Albany NY) |
| ISSN: | 1945-4589 |
| Popis: | The clearance of myelin debris is a critical step in the functional recovery following spinal cord injury (SCI). As phagocytes do, microvascular endothelial cells (MECs) participate in myelin debris clearance at the injury site within one week. Our group has verified that G protein-coupled receptor kinase 2 interacting protein-1 (GIT1) is essential in autophagy and angiogenesis, both of which are tightly related to the uptake and degradation of myelin debris by MECs. Here, we analyzed the performance and mechanism of GIT1 in myelin debris clearance after SCI. The SCI contusion model was established and in vitro MECs were treated with myelin debris. Better recovery from traumatic SCI was observed in the GIT1 WT mice than in the GIT1 KO mice. More importantly, we found that GIT1 prompted MECs to clear myelin debris and further enhanced MECs angiogenesis in vivo and in vitro. Mechanistically, GIT1-mediated autophagy contributed to the clearance of myelin debris by MECs. In this study, we demonstrated that GIT1 may prompt MECs to clear myelin debris via autophagy and further stimulate MECs angiogenesis via upregulating VEGF. Our results indicate that GITI may serve as a promising target for accelerating myelin debris clearance and improving SCI recovery. |
| Databáze: | OpenAIRE |
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