Green Photoluminescent Methodology for Aluminium Traces Quantification in 24-Hour Urine of Subjects with Different Exposition to Tobacco Smoke
| Autor: | Liliana Patricia Fernandez, María Carolina Talio, Debora Gimena Santarossa |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Detection limit
Analyte TOBACCO Chromatography Calibration curve QUINIZARINE Ciencias Químicas chemistry.chemical_element Urine URINE SAMPLES SOLID PHASE EXTRACTION law.invention SOLID SURFACE FLUORESCENCE Matrix (chemical analysis) purl.org/becyt/ford/1 [https] Psychiatry and Mental health chemistry Aluminium law ALUMINIUM TRACES purl.org/becyt/ford/1.4 [https] Química Analítica Solid phase extraction Atomic absorption spectroscopy CIENCIAS NATURALES Y EXACTAS |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| DOI: | 10.4236/ajac.2018.910038 |
| Popis: | The aim of this work has been to evaluate the aluminium Al(III) traces contents in 24-hours urine samples from subjects with different tobacco smoke exposition using a new methodology using 1,4-dihydroxy-9,10-anthraquinone (Quinizarine, QZ) as a fluorosphore. Biological samples were tested using commercial reagent strips and clinical parameters. Al(III) was determined complexing with QZ followed by a solid phase extraction step using Nylon membranes as solid support. The analyte was subsequently quantified by solid surface fluorescence (SSF, λem= 573, λexc= 490) with a detection limit of 0.88 µg L-1 and quantification limit of 2.69 µg L-1. The calibration curve was linear from 2.69 to 499.13 µg L-1 Al(III) (R2 =0.9973). Urine samples were successfully analysed with an average recovery close to 100%. Solid phase extraction step showed efficacy to eliminate foreign ions and the highly fluorescent matrix own of urine. Results were validated by electrothermal atomic absorption spectrometry (ETAAS) with an adequate concordance. The new methodology has low operation cost with simple instrumentation and without organic solvent. Fil: Santarossa, Debora Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina Fil: Talio, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina Fil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química. Área de Química Analítica; Argentina |
| Databáze: | OpenAIRE |
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