Equine blastocyst size affects gene expression

Autor: E Derisoud, L Jouneau, A Margat, C Gourtay, C Dubois, C Archilla, Y Jaszczyszyn, M Dahirel, N Daniel, N Peynot, L Briot, F De Geoffroy, L Wimel, V Duranthon, P Chavatte-Palmer
Přispěvatelé: Biologie de la Reproduction, Environnement, Epigénétique & Développement (BREED), École nationale vétérinaire - Alfort (ENVA)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Français du Cheval et de l'Equitation [Saumur] (IFCE), La Jumenterie du Pin [IFCE], Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Institut Français du Cheval et de l'Equitation, Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Journal of Equine Veterinary Science
Journal of Equine Veterinary Science, 2022, 113 (27), pp.103982. ⟨10.1016/j.jevs.2022.103982⟩
ISSN: 0737-0806
DOI: 10.1016/j.jevs.2022.103982⟩
Popis: International audience; This study aimed to evaluate gene expression for embryos collected 8±1.5 days after ovulation, of different sizes. Non-lactating Saddlebred mares located on two experimental farms were inseminated with semen from one stallion per farm after hCG induction. Mares were examined for ovulation at 24 or 48 h after hCG, depending on the farm. Twenty-eight embryos (11 and 17, respectively) were collected 8 days after ovulation was confirmed, with each embryo from a different dam. Embryos were measured and bisected to obtain trophoblast (TE) or inner cell mass enriched trophoblast (TE-ICM). Paired end, non-oriented RNA sequencing was performed (Illumina, NextSeq500) on both samples of embryos of different diameters: Small (1,200µm, 1,719±488µm, n=8). For TE-ICM data, deconvolution (DeMixT) was used to discriminate gene expression in ICM vs TE. Differential expression was analyzed (DESeq2) with farm and embryo sex as cofactors (false discovery rate (FDR)
Databáze: OpenAIRE
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