Unraveling the genetic basis of xylose consumption in engineered Saccharomyces cerevisiae strains
| Autor: | Gonçalo Amarante Guimarães Pereira, Ludimila Dias Almeida, Guilherme Borelli, Sheila Tiemi Nagamatsu, Nadia M. V. Sampaio, Marcelo Falsarella Carazzolle, Renan A. S. Pirolla, Juan Lucas Argueso, Thamy Lívia Ribeiro Corrêa, Leandro Vieira dos Santos |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Xylose isomerase Heterozygote Saccharomyces cerevisiae Proteins Iron Saccharomyces cerevisiae Karyotype Lignocellulosic biomass Xylose 7. Clean energy 01 natural sciences Polymorphism Single Nucleotide Article Metabolic engineering Evolution Molecular 03 medical and health sciences chemistry.chemical_compound Transformation Genetic 010608 biotechnology Point Mutation Ethanol fuel 2. Zero hunger Multidisciplinary biology Base Sequence Chemistry business.industry Nucleotides Homozygote biology.organism_classification Diploidy Yeast Biotechnology 030104 developmental biology Biochemistry Metabolic Engineering Fermentation Genome Fungal business Genetic Engineering |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep38676 |
| Popis: | The development of biocatalysts capable of fermenting xylose, a five-carbon sugar abundant in lignocellulosic biomass, is a key step to achieve a viable production of second-generation ethanol. In this work, a robust industrial strain of Saccharomyces cerevisiae was modified by the addition of essential genes for pentose metabolism. Subsequently, taken through cycles of adaptive evolution with selection for optimal xylose utilization, strains could efficiently convert xylose to ethanol with a yield of about 0.46 g ethanol/g xylose. Though evolved independently, two strains carried shared mutations: amplification of the xylose isomerase gene and inactivation of ISU1, a gene encoding a scaffold protein involved in the assembly of iron-sulfur clusters. In addition, one of evolved strains carried a mutation in SSK2, a member of MAPKKK signaling pathway. In validation experiments, mutating ISU1 or SSK2 improved the ability to metabolize xylose of yeast cells without adaptive evolution, suggesting that these genes are key players in a regulatory network for xylose fermentation. Furthermore, addition of iron ion to the growth media improved xylose fermentation even by non-evolved cells. Our results provide promising new targets for metabolic engineering of C5-yeasts and point to iron as a potential new additive for improvement of second-generation ethanol production. |
| Databáze: | OpenAIRE |
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