Effects of Streptococcus mutans on dendritic cell activation and function
| Autor: | Jennifer Malcolm, John Butcher, Robert A. Benson, D. M. Deng, Paul Garside, James M. Brewer, Shauna Culshaw |
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| Přispěvatelé: | Preventieve tandheelkunde (OII, ACTA), Preventive Dentistry |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Dental Caries Susceptibility
T-Lymphocytes Genes MHC Class II Antigen presentation Bone Marrow Cells Mice Transgenic Lymphocyte Activation Microbiology Streptococcus mutans Mice Immune system SDG 3 - Good Health and Well-being Antigen Co-stimulation Animals General Dentistry Cells Cultured Antigen Presentation biology Dendritic Cells Dendritic cell Flow Cytometry biology.organism_classification Acquired immune system Coculture Techniques Mice Inbred C57BL Immunology Female Cell activation |
| Zdroj: | Journal of Dental Research, 90(10), 1221-1227. SAGE Publications Inc. Butcher, J P, Malcolm, J, Benson, R A, Deng, D M, Brewer, J M, Garside, P & Culshaw, S 2011, ' Effects of Streptococcus mutans on dendritic cell activation and function ', Journal of Dental Research, vol. 90, no. 10, pp. 1221-1227 . https://doi.org/10.1177/0022034511412970 |
| ISSN: | 0022-0345 |
| DOI: | 10.1177/0022034511412970 |
| Popis: | Despite existing preventive and therapeutic measures, caries remains a ubiquitous infectious disease. Vaccine studies suggest that an adaptive immune response, culminating in effective antibody production, may reduce an individual’s susceptibility to caries. However, the efficacy of the immune response elicited by mutans streptococci in the oral cavity remains controversial. A greater understanding of the early stages of the adaptive immune response to cariogenic bacteria may potentially assist therapeutic targeting and design. We therefore sought to characterize dendritic cell (DC) activation and antigen presentation following Streptococcus mutans exposure. We found that S. mutans up-regulated DC expression of co-stimulatory molecules and MHCII in vitro and that DCs effectively processed and presented exogenously administered antigen. These DCs effectively initiated T-cell proliferation, but this was abrogated by live bacteria. The in vitro DC activation effects were not mirrored in vivo, where DCs in draining lymph nodes did not mature following oral exposure to S. mutans. Analysis of these data provides a model for studying antigen uptake from the oral cavity and evidence that, in vitro, S. mutans activates dendritic cells, a critical event for initiating adaptive immunity. |
| Databáze: | OpenAIRE |
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