Identification and expression of natriuretic peptide receptor type-A and -B mRNA in freshwater and seawater rainbow trout
| Autor: | Mark D. Powell, Scott Nankervis, Janet McLeod, Tes Toop |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
endocrine system
medicine.medical_specialty animal structures Physiology medicine.drug_class animal diseases Molecular Sequence Data Fresh Water Kidney Biochemistry Polymerase Chain Reaction Endocrinology Internal medicine medicine Natriuretic peptide Animals Seawater Amino Acid Sequence RNA Messenger Cloning Molecular Intestinal Mucosa Receptor Peptide sequence Ecology Evolution Behavior and Systematics Regulation of gene expression Messenger RNA biology urogenital system Euryhaline Water-Electrolyte Balance biology.organism_classification Molecular biology Adaptation Physiological Gene Expression Regulation Guanylate Cyclase Oncorhynchus mykiss Freshwater fish Animal Science and Zoology Rainbow trout Receptors Atrial Natriuretic Factor |
| Zdroj: | Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology. 177(2) |
| ISSN: | 0174-1578 |
| Popis: | Natriuretic peptide receptors mediate the physiological response of natriuretic peptide hormones. One of the natriuretic peptide receptor types is the particulate guanylyl cyclase receptors, of which there are two identified: NPR-A and NPR-B. In fishes, these have been sequenced and characterized in eels, medaka, and dogfish shark (NPR-B only). The euryhaline rainbow trout provides an opportunity to further pursue examination of the system in teleosts. In this study, partial rainbow trout NPR-A-like and NPR-B-like mRNA sequences were identified via PCR and cloning. The sequence information was used in real-time PCR to examine mRNA expression in a variety of tissues of freshwater rainbow trout and rainbow trout acclimated to 35 parts per thousand seawater for a period of 10 days. In the excretory kidney and posterior intestine, real-time PCR analysis showed greater expression of NPR-B in freshwater fish than in those adapted to seawater; otherwise, there was no difference in the expression of the individual receptors in fresh water or seawater. In general, the expression of the NPR-A and NPR-B type receptors was quite low. These findings indicate that NPR-A and NPR-B mRNA expression is minimally altered under the experimental regime used in this study. |
| Databáze: | OpenAIRE |
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