Analysis of the cytochrome c oxidase subunit 1 (COX1) gene reveals the unique evolution of the giant panda
| Autor: | Pang Huizhong, Desheng Li, Ye Wang, Yaodong Hu, Chengdong Wang, Zhu Yun, Rongping Wei, Diyan Li, Dan Lan, Hemin Zhang, Ling Shanshan |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Population Evolution Molecular 03 medical and health sciences 0302 clinical medicine biology.animal Genetic variation Genetics Animals Cytochrome c oxidase Selection Genetic education Gene Phylogeny Ailuropoda melanoleuca Genetic diversity education.field_of_study biology Phylogenetic tree General Medicine Protein Subunits 030104 developmental biology Mitochondrial respiratory chain Haplotypes 030220 oncology & carcinogenesis Cyclooxygenase 1 biology.protein Ursidae |
| Zdroj: | Gene. 592:303-307 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/j.gene.2016.07.029 |
| Popis: | As the rate-limiting enzyme of the mitochondrial respiratory chain, cytochrome c oxidase (COX) plays a crucial role in biological metabolism. "Living fossil" giant panda (Ailuropoda melanoleuca) is well-known for its special bamboo diet. In an effort to explore functional variation of COX1 in the energy metabolism behind giant panda's low-energy bamboo diet, we looked at genetic variation of COX1 gene in giant panda, and tested for its selection effect. In 1545 base pairs of the gene from 15 samples, 9 positions were variable and 1 mutation leaded to an amino acid sequence change. COX1 gene produces six haplotypes, nucleotide (pi), haplotype diversity (Hd). In addition, the average number of nucleotide differences (k) is 0.001629±0.001036, 0.8083±0.0694 and 2.517, respectively. Also, dN/dS ratio is significantly below 1. These results indicated that giant panda had a low population genetic diversity, and an obvious purifying selection of the COX1 gene which reduces synthesis of ATP determines giant panda's low-energy bamboo diet. Phylogenetic trees based on the COX1 gene were constructed to demonstrate that giant panda is the sister group of other Ursidae. |
| Databáze: | OpenAIRE |
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