The effect of LED photobiomodulation on the proliferation and osteoblastic differentiation of periodontal ligament stem cells: in vitro
| Autor: | Rochaya Chintavalakorn, Chaiyapol Chaweewannakorn, Rudee Surarit, Hathaitip Sritanaudomchai, Peerapong Santiwong |
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| Rok vydání: | 2021 |
| Předmět: |
Enzyme level
Periodontal ligament stem cells Periodontal Ligament Stem Cells ALIZARIN RED chemistry.chemical_element Cell Differentiation Orthodontics 030206 dentistry Calcium Molecular biology In vitro Staining 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine chemistry Osteogenesis Alkaline phosphatase Statistical analysis Cells Cultured Cell Proliferation |
| Zdroj: | Journal of the World Federation of Orthodontists. 10:79-85 |
| ISSN: | 2212-4438 |
| Popis: | The aim of this study was to investigate the influence of three different light-emitting diode (LED) wavelengths on the proliferation and osteoblastic differentiation of periodontal ligament stem cells (PDLSCs) in vitro.PDLSCs seeded on 96- and 24-well plates, for proliferation and osteoblastic differentiation, respectively, were irradiated daily by LED light with peak emission wavelengths of 630, 680, and 830 nm at constant energy densities of 3.5 J/cmResults obtained from statistical analysis of the experimental data indicated that the rate of proliferation (P0.05) in 830-nm irradiated cultures were significantly higher than the control samples at day 6 and 8; whereas, for the 630- and 680-nm groups, test results showed lower proliferation rates at day 8. For osteoblastic differentiation, significantly greater mineralization than the control samples was detected in the red-light groups (630 and 680 nm) during the late differentiation period (P0.001), which was supported by a higher ALP activity of the 630- and 680-nm groups in the early stage (P0.01).The results of this study demonstrate that the PDLSCs responded differently to specific LED wavelengths. For enhancing cellular proliferation, 830-nm LED irradiation was more effective. On the other hand, the wavelengths of 630 and 680 nm were better for stimulating osteoblastic differentiation. |
| Databáze: | OpenAIRE |
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