Defective Circulating Regulatory B Cells in Patients with Dilated Cardiomyopathy

Autor: Jingyong Li, Jiao Jiao, Ni Xia, Bingjie Lv, Yi-Qiu Wang, Ke-Jing Wang, Shaofang Nie, Shuang Wen, Yuzhi Lu, Yuhua Liao, Xingdi Zhou, Xiang Cheng, Tingting Tang
Rok vydání: 2018
Předmět:
CD4-Positive T-Lymphocytes
Male
0301 basic medicine
Physiology
T-Lymphocytes
Regulatory
lcsh:Physiology
Pathogenesis
0302 clinical medicine
Natriuretic Peptide
Brain
Medicine
lcsh:QD415-436
IL-2 receptor
B-Lymphocytes
Regulatory
Ejection fraction
lcsh:QP1-981
medicine.diagnostic_test
Cell Differentiation
Dilated cardiomyopathy
Middle Aged
Interleukin-10
medicine.anatomical_structure
Female
Dilated Cardiomyopathy (DCM)
Adult
Cardiomyopathy
Dilated
Heart Ventricles
Regulatory B cells
CD24hiCD27+ B cell
Flow cytometry
lcsh:Biochemistry
03 medical and health sciences
Immune system
Humans
cardiovascular diseases
Regulatory B cell (Breg)
B cell
Aged
Cell Proliferation
Tumor Necrosis Factor-alpha
business.industry
CD24 Antigen
medicine.disease
Peptide Fragments
Tumor Necrosis Factor Receptor Superfamily
Member 7
030104 developmental biology
Case-Control Studies
Immunology
Leukocytes
Mononuclear
business
030215 immunology
Zdroj: Cellular Physiology and Biochemistry, Vol 46, Iss 1, Pp 23-35 (2018)
ISSN: 1421-9778
1015-8987
DOI: 10.1159/000488405
Popis: Background/Aims: Newly identified IL-10-producing regulatory B cells (Bregs) have been shown to play an important role in the suppression of immune responses. Chronic immune activation participates in the pathogenesis of dilated cardiomyopathy (DCM) but whether Bregs are involved in its development remains unclear. We aimed to investigate the circulating frequency and function of Bregs in DCM. Methods: In total, 35 DCM patients (20 men and 15 women) and 44 healthy controls (23 men and 21 women) were included in the experiment, and the frequency of Bregs was detected using flow cytometry. Results: According to our results, the frequency of circulating IL-10-producing Bregs was significantly lower in DCM patients compared with healthy controls. Furthermore, the CD24hiCD27+ B cell subset in which IL-10-producing Bregs were mainly enriched from DCM patients showed impaired IL-10 expression and a decreased ability to suppress the TNF-α production of CD4+CD25- Tconv cells and to maintain Tregs differentiation. Correlation analysis showed that the frequency of IL-10-producing Bregs and the suppressive function of CD24hiCD27+ B cells were positively correlated with left ventricular ejection fraction and negatively correlated with NT-proBNP in DCM patients. Conclusions: In conclusion, the reduced frequency and impaired functions suggest a potential role of Bregs in the development of DCM.
Databáze: OpenAIRE
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