The adherens junction–associated LIM domain protein Smallish regulates epithelial morphogenesis
| Autor: | Paulina Hordowska, Irina Peek, Maria Leptin, Silke Wiesner, Parisa Kakanj, Fabian A. Renschler, Stefan Luschnig, Andreas Ramrath, Mona Honemann-Capito, Hamze Beati, Andreas Wodarz, Jade Glashauser |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
animal structures Morphogenesis Biology Article Adherens junction 03 medical and health sciences Embryonic morphogenesis Animals Drosophila Proteins Research Articles Protein kinase C LIM domain Epidermis (botany) Epithelial Cells Apical constriction Adherens Junctions Cell Biology Embryonic stem cell Cell biology Drosophila melanogaster 030104 developmental biology Epidermis |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| DOI: | 10.1083/jcb.201610098 |
| Popis: | Cell–cell adhesion and cell shape are regulated at adherens junctions during embryonic morphogenesis. Beati et al. show that the Drosophila LIM domain protein Smallish interacts with Bazooka, Canoe, and Src42A at adherens junctions. Loss-of-function and gain-of-function phenotypes reveal a function for Smallish in regulation of actomyosin contractility and cell shape. In epithelia, cells adhere to each other in a dynamic fashion, allowing the cells to change their shape and move along each other during morphogenesis. The regulation of adhesion occurs at the belt-shaped adherens junction, the zonula adherens (ZA). Formation of the ZA depends on components of the Par–atypical PKC (Par-aPKC) complex of polarity regulators. We have identified the Lin11, Isl-1, Mec-3 (LIM) protein Smallish (Smash), the orthologue of vertebrate LMO7, as a binding partner of Bazooka/Par-3 (Baz), a core component of the Par-aPKC complex. Smash also binds to Canoe/Afadin and the tyrosine kinase Src42A and localizes to the ZA in a planar polarized fashion. Animals lacking Smash show loss of planar cell polarity (PCP) in the embryonic epidermis and reduced cell bond tension, leading to severe defects during embryonic morphogenesis of epithelial tissues and organs. Overexpression of Smash causes apical constriction of epithelial cells. We propose that Smash is a key regulator of morphogenesis coordinating PCP and actomyosin contractility at the ZA. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|