Bracoviruses recruit host integrases for their integration into caterpillar’s genome
| Autor: | Wang Zehua, Xiaotong Wu, Jiachen Zhu, Yuenan Zhou, Ting Chen, Elisabeth Huguet, Rong-min Hu, Chen Xuexin, Ye Xiqian, Jean-Michel Drezen, Huang Jianhua, Min Shi |
|---|---|
| Přispěvatelé: | Zhejiang University, Institut de recherche sur la biologie de l'insecte UMR7261 (IRBI), Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Université de Tours-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Cancer Research
Life Cycles Hemocytes Wasps Moths QH426-470 Genome chemistry.chemical_compound White Blood Cells Medical Conditions Larvae Animal Cells Invertebrate Genomics Medicine and Health Sciences Cotesia vestalis Genetics (clinical) Genetic Interference Caterpillars Genetics 0303 health sciences biology Polydnavirus 030302 biochemistry & molecular biology Eukaryota Genomics 3. Good health Insects Moths and Butterflies [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Cellular Types Bracovirus Research Article food.ingredient Arthropoda Immune Cells Immunology Human Genomics Host-Parasite Interactions 03 medical and health sciences food Parasitic Diseases Animals Molecular Biology Ecology Evolution Behavior and Systematics 030304 developmental biology Blood Cells Integrases Host (biology) Organisms Biology and Life Sciences Cell Biology biology.organism_classification Invertebrates [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology chemistry Animal Genomics Polydnaviridae DNA Viral Zoology Entomology DNA Developmental Biology |
| Zdroj: | PLoS Genetics PLoS Genetics, Public Library of Science, 2021, 17 (9), pp.e1009751. ⟨10.1371/journal.pgen.1009751⟩ PLoS Genetics, Vol 17, Iss 9, p e1009751 (2021) |
| ISSN: | 1553-7390 1553-7404 |
| DOI: | 10.1371/journal.pgen.1009751⟩ |
| Popis: | Some DNA viruses infect host animals usually by integrating their DNAs into the host genome. However, the mechanisms for integration remain largely unknown. Here, we find that Cotesia vestalis bracovirus (CvBV), a polydnavirus of the parasitic wasp C. vestalis (Haliday), integrates its DNA circles into host Plutella xylostella (L.) genome by two distinct strategies, conservatively and randomly, through high-throughput sequencing analysis. We confirmed that the conservatively integrating circles contain an essential “8+5” nucleotides motif which is required for integration. Then we find CvBV circles are integrated into the caterpillar’s genome in three temporal patterns, the early, mid and late stage-integration. We further identify that three CvBV-encoded integrases are responsible for some, but not all of the virus circle integrations, indeed they mainly participate in the processes of early stage-integration. Strikingly, we find two P. xylostella retroviral integrases (PxIN1 and PxIN2) are highly induced upon wasp parasitism, and PxIN1 is crucial for integration of some other early-integrated CvBV circles, such as CvBV_04, CvBV_12 and CvBV_24, while PxIN2 is important for integration of a late-integrated CvBV circle, CvBV_21. Our data uncover a novel mechanism in which CvBV integrates into the infected host genome, not only by utilizing its own integrases, but also by recruiting host enzymes. These findings will strongly deepen our understanding of how bracoviruses regulate and integrate into their hosts. Author summary Parasitoid wasps are a very large group of animals that live in or on the other arthropods. Polydnaviruses (PDVs) are a special kind of DNA virus associated parasitic wasps (primary hosts), which do not replicate in their infected caterpillar hosts (secondary hosts). During parasitoid oviposition, PDVs enter infected hosts and integrate their DNA into the genome of infected hosts, then their virulence genes begin to express and manipulate multiple biological process of hosts to fulfill all the requirements of parasitoids offspring. However, the mechanisms for integration remain largely unknown. Our research found the integration of PDVs is not only by utilizing its own integrases, but also by recruiting host enzymes. Here, we provide a new concept that host intergrases are involved in the integration of PDVs into host genome, and it greatly enhance our understanding of how PDVs regulate and integrate into their hosts, and may also inspire studies on how some DNA viruses may integrate their DNAs into human genome. |
| Databáze: | OpenAIRE |
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