Diversity of Bacterial Communities Associated with the Indian Ocean Sponge Tsitsikamma favus That Contains the Bioactive Pyrroloiminoquinones, Tsitsikammamine A and B
| Autor: | Michael T. Davies-Coleman, Fan Zhang, Russell T. Hill, Gwynneth F. Matcher, Tara A. Walmsley, Rosemary A. Dorrington |
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| Rok vydání: | 2012 |
| Předmět: |
education.field_of_study
biology Clavelina Ecology Microbial Consortia Population Biodiversity Betaproteobacteria Zoology biology.organism_classification 16S ribosomal RNA Applied Microbiology and Biotechnology Porifera Sponge Species Specificity Quinolines Animals Pyrosequencing Pyrroles education Indian Ocean Temperature gradient gel electrophoresis Pyrroloiminoquinones |
| Zdroj: | Marine Biotechnology. 14:681-691 |
| ISSN: | 1436-2236 1436-2228 |
| DOI: | 10.1007/s10126-012-9430-y |
| Popis: | Tsitsikamma favus is a latrunculid sponge endemic to the coast of South Africa that produces unique pyrroloiminoquinones known as tsitsikammamines. Wakayin and makaluvamine A are structurally similar to the tsitsikammamines and are the only pyrroloiminoquinones isolated from a source other than Porifera (namely a Fijian ascidian Clavelina sp. and a laboratory culture of the myxomycete Didymium bahiense, respectively). The source of the tsitsikammamines is hypothesised to be microbial, which could provide a means of overcoming the current supply problem. This study focuses on characterising the microbial diversity associated with T. favus. We have used denaturing gradient gel electrophoresis together with clonal and deep sequencing of microbial 16S rRNA gene amplicons to show that specimens of this sponge species contain a distinct and conserved microbial population, which is stable over time and is dominated by a unique Betaproteobacterium species. |
| Databáze: | OpenAIRE |
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