Construction of a Llama Bacterial Artificial Chromosome Library with Approximately 9-Fold Genome Equivalent Coverage
| Autor: | J. Genho, S. Blumell, K. W. Airmet, J.D. Hinckley ., M. Lehnardt, M. Moss, M. Weed, L. T. Tree, M. R. Stevens, David L. Kooyman, R. Theodosis, A. K. Gustafson, K. Ulicny |
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| Rok vydání: | 2012 |
| Předmět: |
Chromosomes
Artificial Bacterial Article Subject lcsh:Biotechnology Health Toxicology and Mutagenesis Companion animal lcsh:Medicine Biology Genome Insert (molecular biology) Genome Size lcsh:TP248.13-248.65 Genetics Animals Humans Molecular Biology Genome size Gene Library Bacterial artificial chromosome lcsh:R Genomics General Medicine Molecular Medicine Camelids New World Research Article Biotechnology |
| Zdroj: | Journal of Biomedicine and Biotechnology Journal of Biomedicine and Biotechnology, Vol 2012 (2012) |
| ISSN: | 1110-7251 1110-7243 |
| Popis: | The Ilama is an important agricultural livestock in much of South America. The llama is increasing in popularity in the United States as a companion animal. Little work has been done to improve llama production using modern technology. A paucity of information is available regarding the llama genome. We report the construction of a llama bacterial artificial chromosome (BAC) library of about 196,224 clones in the vector pECBAC1. Using flow cytometry and bovine, human, mouse, and chicken as controls, we determined the llama genome size to be2.4×109 bp. The average insert size of the library is 137.8 kb corresponding to approximately 9-fold genome coverage. Further studies are needed to further characterize the library and llama genome. We anticipate that this new library will help facilitate future genomic studies in the llama. |
| Databáze: | OpenAIRE |
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