Molecular characterization of Trypanosoma evansi isolates from water buffaloes (Bubalus bubalis) in the Philippines
| Autor: | Windell L. Rivera, Claro N. Mingala, Marjo V. Villareal |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Trypanosoma
Veterinary medicine Buffaloes Philippines Molecular Sequence Data Polymerase Chain Reaction Disease Outbreaks law.invention Trypanosomiasis law parasitic diseases Genetic variation Prevalence Animals Internal transcribed spacer Phylogeny Polymerase chain reaction Genetic diversity biology Genetic Variation Outbreak DNA Protozoan Trypanosoma evansi biology.organism_classification Parasitology Bubalus |
| Zdroj: | Acta Parasitologica. 58 |
| ISSN: | 1896-1851 1230-2821 |
| DOI: | 10.2478/s11686-013-0110-5 |
| Popis: | Trypanosoma evansi infection in the Philippines is frequently reported to affect the country’s livestock, particularly, the buffaloes. To assess the prevalence and intraspecific diversity of T. evansi in the country, blood samples from water buffaloes in different geographical regions were collected during an outbreak. T. evansi was detected in all 79 animals tested using PCR targeting the RoTat 1.2 VSG gene. Sequencing of the rDNA complete internal transcribed spacer (ITS) region including the 5.8S subunit showed high similarity (99–100%) between Philippine isolates and known T. evansi isolates in Genbank. Tree construction based on the same region confirmed the close relationship between Philippine and reported Thai isolates as compared to Egyptian isolates separated by relatively small genetic distances, 47 polymorphisms, despite the clustering in four branches. Overall, the results of this study prove genetic diversity within T. evansi species despite previous reports on limited heterogeneity among isolates worldwide. |
| Databáze: | OpenAIRE |
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