Cytotoxic evaluation of sodium hypochlorite, using Pisum sativum L as effective bioindicator
| Autor: | Carlos Andrés Torres-León, Jhan Piero Rojas-Suárez, Seir Antonio Salazar-Mercado |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Mitotic index
Sodium Hypochlorite Cytotoxicity Health Toxicology and Mutagenesis 0211 other engineering and technologies Mitosis 02 engineering and technology 010501 environmental sciences Models Biological Plant Roots 01 natural sciences Pisum chemistry.chemical_compound Sativum P. sativum Mitotic Index Cytotoxic T cell 0105 earth and related environmental sciences Cell Nucleus Chromosome Aberrations 021110 strategic defence & security studies Environmental Biomarkers biology Chemistry Peas Public Health Environmental and Occupational Health General Medicine biology.organism_classification Pollution Molecular biology Sodium hypochlorite Distilled water Cellular abnormalities Toxicity Micronucleus test |
| Zdroj: | Repositorio Digital UFPS UNIVERSIDAD FRANCISCO DE PAULA SANTANDER instacron:Universidad Francisco de Paula Santander |
| ISSN: | 0147-6513 |
| Popis: | The objective of this study was to evaluate the cytotoxic effect of different sodium hypochlorite concentrations, using apical root cells of P. sativum as a bioindicator. Initially, the seeds of P. sativum were exposed to different concentrations of sodium hypochlorite (0.1, 0.2, 0.4, 0.8, 1.6, 2 mg L−1) and to a control solution based on distilled water. Next, root growth was measured during 24, 48 and 72 h. Subsequently, the mitotic index (MI) and cellular anomalies (5000 cells per treatment) were determined at 72 h. According to the results obtained, a decrease in root growth was observed at concentrations of 0.4, 1.6 and 2 mg L−1. Likewise, it was evident that, among all the evaluated concentrations, an inhibition of mitosis higher than 50% was presented. Additionally, chromosomal anomalies were also generated, such as Nuclear notch, lagging chromosomes and Chromosomal break, which were present in all the concentrations evaluated. In addition, the presence of micronuclei at concentrations of 2.0 and 1.6 mg L−1 indicate that sodium hypochlorite is a highly cytotoxic substance. Therefore, P. sativum is a specie that offers a feasible experimental model to be implemented in the laboratory with the aim to evaluate the cytotoxic effect of any cytotoxic substance. |
| Databáze: | OpenAIRE |
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