Decrease in peripheral type 1 over type 2 T cell cytokine production in patients with rheumatoid arthritis correlates with an increase in severity of disease
| Autor: | F. H. J. Gmelig-Meyling, J.A. van Roon, F.P.J.G. Lafeber, J. W. J. Bijlsma, C. M. Verhoef, J. L. A. M. Van Roy, O. Huber-Bruning |
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| Přispěvatelé: | Other departments |
| Rok vydání: | 1998 |
| Předmět: |
Male
medicine.medical_treatment T cell Immunology Arthritis Blood Sedimentation Lymphocyte Activation Peripheral blood mononuclear cell General Biochemistry Genetics and Molecular Biology Statistics Nonparametric Extended Reports Arthritis Rheumatoid Interferon-gamma Th2 Cells Rheumatology Osteoarthritis medicine Immunology and Allergy Humans Interferon gamma Arthrography Interleukin 4 Aged Aged 80 and over business.industry Tumor Necrosis Factor-alpha Middle Aged Th1 Cells medicine.disease T cell cytokine production Cytokine medicine.anatomical_structure C-Reactive Protein Rheumatoid arthritis Cytokines Regression Analysis Female Interleukin-4 business medicine.drug |
| Zdroj: | Annals of the rheumatic diseases, 56(11), 656-660. BMJ Publishing Group |
| ISSN: | 0003-4967 |
| Popis: | Objectives—To compare peripheral type 1 (T1) and type 2 (T2) T cell activities in rheumatoid arthritis (RA) patients with that found for osteoarthritic (OA) patients and healthy controls and to correlate peripheral T1/T2 cell activity in RA with parameters of the disease. METHODS—Peripheral blood mononuclear cells were isolated from patients with RA (n=66), OA (n=19), and healthy controls (n=15). Primary T cell activity in these mononuclear cells was enhanced by means of anti-CD3/anti-CD28, which mimicks stimulation of T cells by activation of the T cell receptor and a major co-stimulatory signal. Interferon gamma (IFNγ) production and interleukin 4 (IL4) production in the three groups were quantified as measures of T1 and T2 cell activity, respectively, and compared. Serum tumour necrosis factor α (TNFα), erythrocyte sedimentation rate (ESR), C reactive protein (CRP), and joint destruction assessed radiographically of RA patients were determined as parameters of disease activity and correlated with T1/T2 cell activity. RESULTS—Peripheral T cells from RA patients produced significantly less IFNγ and more IL4 than T cells from both age and sex matched OA patients and healthy controls. Moreover, in RA patients both a decrease in IFNγ and an increase in IL4 production correlated with an increase in serum TNFα, ESR, CRP, and joint destruction. Conclusions—These results suggest a role for differential T cell activity in RA. In view of the intra-articular T1 cell predominance the results might be explained by selective T1 cell migration into the joint or peripheral suppression of T1 cell activity. |
| Databáze: | OpenAIRE |
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