Chromo-fluorogenic probes for β-galactosidase detection
| Autor: | Beatriz Lozano-Torres, Ramón Martínez-Máñez, Félix Sancenón, Juan F. Blandez |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
In vitro and in vivo detection
02 engineering and technology Cellular senescence 01 natural sciences Biochemistry Analytical Chemistry Residue (chemistry) QUIMICA ORGANICA In vivo beta-Galactosidase detection Beta-Galactosidase detection Animals Humans Monosaccharide Cellular Senescence Enzyme Assays Fluorescent Dyes chemistry.chemical_classification Reporter gene Chemistry Hydrolysis QUIMICA INORGANICA 010401 analytical chemistry Galactose Galactosides Glycosidic bond beta-Galactosidase 021001 nanoscience & nanotechnology In vitro 0104 chemical sciences Enzyme Molecular Probes Chromo-fluorogenic probes Colorimetry 0210 nano-technology Molecular probe |
| Zdroj: | ANALYTICAL AND BIOANALYTICAL CHEMISTRY r-CIPF. Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF) instname RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia r-IIS La Fe. Repositorio Institucional de Producción Científica del Instituto de Investigación Sanitaria La Fe r-CIPF: Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF) Centro de Investigación Principe Felipe (CIPF) |
| ISSN: | 1618-2650 1618-2642 |
| Popis: | [EN] beta-Galactosidase (beta-Gal) is a widely used enzyme as a reporter gene in the field of molecular biology which hydrolyzes the beta-galactosides into monosaccharides. beta-Gal is an essential enzyme in humans and its deficiency or its overexpression results in several rare diseases. Cellular senescence is probably one of the most relevant physiological disorders that involve beta-Gal enzyme. In this review, we assess the progress made to date in the design of molecular-based probes for the detection of beta-Gal both in vitro and in vivo. Most of the reported molecular probes for the detection of beta-Gal consist of a galactopyranoside residue attached to a signalling unit through glycosidic bonds. The beta-Gal-induced hydrolysis of the glycosidic bonds released the signalling unit with remarkable changes in color and/or emission. Additional examples based on other approaches are also described. The wide applicability of these probes for the rapid and in situ detection of de-regulation beta-Gal-related diseases has boosted the research in this fertile field R.M laboratory members received the financial support from the Spanish Government (project RTI2018-100910-B-C41) and the Generalitat Valenciana (project PROMETEO 2018/024). B.L-T. received support from the Spanish Ministry of Economy for their PhD grants (FPU15/02707). J. F.-B received fellowship (CD19/00038) |
| Databáze: | OpenAIRE |
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