Ultrastructural characteristics of a cell line derived from a melanotic neuroectodermal tumor of infancy
| Autor: | Barry Steinberg, Charles F. Shuler, David Stetson, Lewis Claman |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Male
Cancer Research Pathology medicine.medical_specialty Cell type Population Coated vesicle Biology Microfilament Cell Line Pathology and Forensic Medicine Tumor Cells Cultured medicine Freeze Fracturing Humans education Maxillary Neoplasms education.field_of_study Melanotic neuroectodermal tumor of infancy Endoplasmic reticulum Infant Neoplasms Germ Cell and Embryonal medicine.disease Microscopy Electron Otorhinolaryngology Cytoplasm Ultrastructure Melanocytes Periodontics Oral Surgery |
| Zdroj: | Journal of Oral Pathology and Medicine. 20:245-249 |
| ISSN: | 1600-0714 0904-2512 |
| DOI: | 10.1111/j.1600-0714.1991.tb00428.x |
| Popis: | Thin section and freeze-fracture transmission electron microscopy were used to examine and identify the cytoplasmic and membrane structures in a cell line derived from a melanotic neuroectodermal tumor of infancy (MNTI). The cultured cells had a uniform appearance after 70 population doublings characterized by long dendritic processes and evidence of melanin production. The cytoplasm contained numerous melanosomes in various stages of development, vesiculated rough endoplasmic reticulum, microfilaments and uncoated as well as coated vesicles. The membrane specializations included caveoli, coated pits, gap junctions, microfilaments, desmosome-like structures and lamellipodia. The ultrastructural appearance of the cultured MNTI cells was similar to features previously seen in electron micrographs of MNTI tumor specimens. However, correlated freeze-fracture and thin section micrographs permitted further identification of structures previously described. The MNTI cell line represents one of the cell types of the tumor and provides an opportunity for further study of the pathogenesis of this rare tumor. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text