Anchored cell analysis/sorting coupled with the scrape‐loading/dye‐transfer technique to quantify inhibition of gap‐junctional intercellular communication in WB‐F344 cells by 2,2',4,4' ,5,5'‐hexabromobiphenyl
| Autor: | Mohamed H. El-Fouly, Mark G. Evans, James E. Trosko, Stuart D. Sleight |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Male
Polybrominated Biphenyls Fluorescence spectrometry Analytical chemistry Cell Communication In Vitro Techniques Biology Toxicology Cell junction chemistry.chemical_compound In vivo Animals Lucifer yellow Gap junction Isoquinolines Pollution Rats Inbred F344 In vitro Rats Intercellular Junctions Microscopy Fluorescence chemistry Cell culture Carcinogens Biophysics Intracellular |
| Zdroj: | Journal of Toxicology and Environmental Health. 24:261-271 |
| ISSN: | 0098-4108 |
| DOI: | 10.1080/15287398809531159 |
| Popis: | Inhibition of intercellular communication has been hypothesized to play a role in tumor promotion. The compound 2,2',4,4',5,5'-hexabromobiphenyl (245-HBB) is a tumor promoter in vivo and blocks intercellular communication in vitro. The scrape-loading/dye-transfer (SL/DT) assay was used to assess this in vitro effect at varying concentrations of 245-HBB. The SL/DT technique is based on the intracellular loading of a fluorescent dye, lucifer yellow (LY), and monitoring its transfer into adjacent cells via patent gap junctions. Confluent WB-F344 (rat epithelial) cells were exposed to various noncytolethal concentrations of 245-HBB. Transfer of LY was then quantified with anchored cell analysis/sorting (ACAS 470, Meridian Instruments, Okemos, Mich.). The results indicate an inverse correlation between the degree of fluorescence in secondary LY-recipient cells and the treatment concentration. The coupling of these two new methods of cellular biology provided rapid quantitative analysis of dye transfer in measuring the concentration/response of modulation of gap-junctional permeability in cultured cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|