Fast and versatile analysis of liposome encapsulation efficiency by nanoParticle exclusion chromatography
| Autor: | Jessica Lin, Nanzhi Zang, Juan Bian, Ponien Kou, Elizabeth S. Levy, Kelly Zhang, Yuchen Fan, James Girotti, Jason Gruenhagen, Vijay Sethuraman |
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| Rok vydání: | 2021 |
| Předmět: |
Drug
Liposome Biodistribution Aqueous solution Chromatography Chemistry media_common.quotation_subject Organic Chemistry Nanoparticle General Medicine Reversed-phase chromatography Biochemistry High-performance liquid chromatography Analytical Chemistry Drug Delivery Systems Drug delivery Liposomes Chromatography Gel Nanoparticles Tissue Distribution media_common |
| Zdroj: | Journal of chromatography. A. 1662 |
| ISSN: | 1873-3778 |
| Popis: | Liposomes are an attractive drug delivery platform for a wide variety of pharmaceutical molecules. Encapsulation efficiency, which refers to the amount of drug contained inside liposomes compared with the total amount of drug, is a critical quality attribute of liposome products, as the free drug in a liposomal formulation may cause toxicity or undesired biodistribution. The determination of encapsulation efficiency requires the measurement of at least two of the three drug populations: total drug, encapsulated drug and free drug. However, direct measurement of the encapsulated drug and free drug remains a challenging analytical task. Nanoparticle exclusion chromatography (nPEC), an emerging high-performance liquid chromatography (HPLC) technique, has shown great potential in separating and quantifying the free drug in liposomal formulations. In this study, nPEC was systematically evaluated for two representative liposomal formulations containing either hydrophilic or hydrophobic small molecule drugs. It is reported for the first time that the insoluble free drug suspended in the aqueous formulation can be directly measured by nPEC. This free drug in the suspension sample was quantified with excellent accuracy and precision. On the other hand, the total drug measurement from dissociated liposomes was confirmed by the benchmark methodology of reversed phase liquid chromatography (RPLC). The facile quantitation of free and total drug in the liposome formulation enables the fast and accurate determination of the encapsulation efficiency, which can be used to guide the formulation development and characterize the product quality. |
| Databáze: | OpenAIRE |
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