RNA‐Seq analysis of potential lncRNAs and genes for the anti‐renal fibrotic effect of norcantharidin
| Autor: | Zheng Xiao, Hong Liu, Jun Li, Hanwen Luo, Ying Li, Dong Zeng, Ying-Jun Liao, Lin Sun, Heng-Ting Xiao, Chengyuan Tang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Apoptosis Biology urologic and male genital diseases Biochemistry Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Renal fibrosis Animals Gene Regulatory Networks RNA-Seq STAT3 Molecular Biology Gene Cells Cultured Tissue homeostasis Cell Proliferation Kidney Norcantharidin urogenital system Cell Biology Bridged Bicyclo Compounds Heterocyclic medicine.disease Fibrosis Phenotype Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation chemistry 030220 oncology & carcinogenesis biology.protein Cancer research RNA Long Noncoding Biomarkers Ureteral Obstruction Kidney disease |
| Zdroj: | Journal of Cellular Biochemistry. 120:17354-17367 |
| ISSN: | 1097-4644 0730-2312 |
| DOI: | 10.1002/jcb.28999 |
| Popis: | Background As a common phenotype in chronic kidney disease, renal interstitial fibrosis has been largely studied. Norcantharidin (NCTD), a derivative of naturally occurring cantharidin, has an anti-renal fibrotic effect. However, its underlying mechanisms of the protective role remain largely unknown. Long noncoding RNAs (lncRNAs) play vital parts in tissue homeostasis modulation under pathophysiological conditions. In this study, we discovered the underlying lncRNAs and genes, which may contribute to the anti-renal fibrotic effects of NCTD. Methods RNA-seq analysis was performed to evaluate profiling of lncRNAs and messenger RNAs (mRNAs) in kidney tissues of sham-control, and unilateral ureteral obstruction (UUO) mouse models with or without NCTD treatment. Systematic bioinformatic analysis of expression levels was used in lncRNAs and mRNAs of NCTD-treated UUO kidneys. Altered expression of lncRNAs and mRNAs levels was confirmed by quantitative real-time polymerase chain reaction analysis. Results 467 lncRNAs and 1502 mRNAs were differentially expressed between UUO- and sham-operated kidneys, and notably, these alterations in UUO-operated kidney were partially reversed following NCTD treatment. Interestingly, the up-regulation of lncRNA Gm16076, Gm26669, and down-regulation of Fam120aos were highly correlated with the up-regulation of mRNA levels of fibrosis-related gene ITGB1, STAT3 and reduction of Pink1 in UUO kidney, respectively. Conclusions The result suggested lncRNAs-regulated genes may contribute to the anti-renal fibrotic effect under NCTD treatment, and thus targeting lncRNAs-controlled genes and their related molecular signaling pathways may serve as a promising therapeutic target in renal fibrosis treatment. |
| Databáze: | OpenAIRE |
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