Variation of crustacean hyperglycemic hormone (cHH) level in the eyestalk and haemolymph of the shrimpPalaemon elegansfollowing stress
| Autor: | Paolo Edomi, Piero Giulio Giulianini, Enrico A. Ferrero, Simonetta Lorenzon, Romina Mettulio |
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| Přispěvatelé: | Edomi, Paolo, Giulianini, PIERO GIULIO, Ferrero, Enrico, Lorenzon, S., Mettulio, R. |
| Rok vydání: | 2004 |
| Předmět: |
Blood Glucose
Lipopolysaccharides medicine.medical_specialty Invertebrate Hormones Physiology Lipopolysaccharide Enzyme-Linked Immunosorbent Assay Nerve Tissue Proteins Aquatic Science Palaemon elegans Arthropod Proteins chemistry.chemical_compound Crustacea Metals Heavy Internal medicine Hemolymph Mediterranean Sea medicine Animals cHH Glucose Heavy metals Water quality Molecular Biology Ecology Evolution Behavior and Systematics Dose-Response Relationship Drug biology Decapoda biology.organism_classification Shrimp Eyestalk Dose–response relationship Heavy metal Endocrinology chemistry Insect Science Biological Assay Animal Science and Zoology Invertebrate hormone Palaemonidae Toxicant |
| Zdroj: | Journal of Experimental Biology. 207:4205-4213 |
| ISSN: | 1477-9145 0022-0949 |
| Popis: | SUMMARYThis study investigates (by means of bioassays and ELISA using an antibody against recombinant cHH) the variation of cHH levels in the eyestalks and haemolymph of Palaemon elegans (Decapoda, Caridea) following exposure to various stresses (heavy metals and lipopolysaccharide), and correlates them with the variation in amount and time course of blood glucose. The dose-relationship between exposure to copper and quick release of cHH from the eyestalk into haemolymph was confirmed by variation of blood glucose with a dose-related hyperglycaemia, that peaked 2 h after immersion in contaminated seawater. Animals exposed to a sublethal concentration of mercury showed the same dose relation between toxicant, release of cHH from the eyestalk,increment of circulating hormone level and subsequent hyperglycaemia as observed for copper contamination. It is of note that although the highest lethal mercury concentration induced the release of cHH from the eyestalk into the haemolymph, it was not followed by a significant variation of blood glucose. Step doses of a bacterial contaminant [such as lipopolysaccharide(LPS) from E. coli injected into shrimps] confirmed the dose-relationship and convergent chain of events that bring about hyperglycaemia. These are the first data that relate the release of cHH from the eyestalk, the circulating hormone level and the consequent glycaemic response to stress. Moreover, they confirm the dose-related pathway that leads to variation of blood glucose as a quantitative biomarker of environmental quality, even at sublethal toxicant concentrations. |
| Databáze: | OpenAIRE |
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