DNA-Templated Protein Arrays for Single-Molecule Imaging
| Autor: | Roslin J. Adamson, Anthony Watts, Helen Attrill, Robert J.C. Gilbert, Alan D. Goddard, Andrew J. Turberfield, Daniele N. Selmi |
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| Rok vydání: | 2011 |
| Předmět: |
Chemistry
Cryo-electron microscopy Mechanical Engineering Protein Array Analysis Molecular Probe Techniques Bioengineering Image processing DNA General Chemistry Condensed Matter Physics Single Molecule Imaging Equipment Failure Analysis chemistry.chemical_compound Template Biophysics Nanotechnology Molecule General Materials Science Self-assembly Macromolecule |
| Zdroj: | Nano Letters. 11:657-660 |
| ISSN: | 1530-6992 1530-6984 |
| DOI: | 10.1021/nl1037769 |
| Popis: | Single-particle electron cryomicroscopy permits structural characterization of noncrystalline protein samples, but throughput is limited by problems associated with sample preparation and image processing. Three-dimensional density maps are reconstructed from high resolution but noisy images of individual molecules. We show that self-assembled DNA nanoaffinity templates can create dense, nonoverlapping arrays of protein molecules, greatly facilitating data collection. We demonstrate this technique using a G-protein-coupled membrane receptor, a soluble G-protein, and a signaling complex of both molecules. |
| Databáze: | OpenAIRE |
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