Evaluation of 11 SARS-CoV-2 antibody tests by using samples from patients with defined IgG antibody titers
| Autor: | Shaman Muradrasoli, Mikaela Olausson, Torbjörn Kjerstadius, Nina Lagerqvist, Johanna Repo, Mia Brytting, Jenny Eriksson, Jonas Klingström, Kimia T Maleki, Thomas Åkerlund, Tor Monsen, Mattias Forsell, Julia Wigren Byström, Charlotta Nilsson, Jenny Verner-Carlsson, Joakim Dillner, Sandra Muschiol, Gordana Bogdanovic, Joel Ljunggren, Åsa Szekely Björndal |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Male Time Factors Infektionsmedicin Antibodies Viral 0302 clinical medicine Medicine 030212 general & internal medicine Child Aged 80 and over Multidisciplinary biology medicine.diagnostic_test Antibody titer Diagnostic test Middle Aged Titer Child Preschool Female Antibody Adult Infectious Medicine Coronavirus disease 2019 (COVID-19) Adolescent Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Science Immunology Immunofluorescence Microbiology Sensitivity and Specificity Article COVID-19 Serological Testing 03 medical and health sciences Young Adult Humans Symptom onset Aged business.industry SARS-CoV-2 Immunology in the medical area COVID-19 030104 developmental biology Immunologi inom det medicinska området Immunoglobulin G biology.protein business |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-8 (2021) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | We evaluated the performance of 11 SARS-CoV-2 antibody tests using a reference set of heat-inactivated samples from 278 unexposed persons and 258 COVID-19 patients, some of whom contributed serial samples. The reference set included samples with a variation in SARS-CoV-2 IgG antibody titers, as determined by an in-house immunofluorescence assay (IFA). The five evaluated rapid diagnostic tests had a specificity of 99.0% and a sensitivity that ranged from 56.3 to 81.6% and decreased with low IFA IgG titers. The specificity was > 99% for five out of six platform-based tests, and when assessed using samples collected ≥ 22 days after symptom onset, two assays had a sensitivity of > 96%. These two assays also detected samples with low IFA titers more frequently than the other assays. In conclusion, the evaluated antibody tests showed a heterogeneity in their performances and only a few tests performed well with samples having low IFA IgG titers, an important aspect for diagnostics and epidemiological investigations. |
| Databáze: | OpenAIRE |
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