The Cellular Inhibitor of Apoptosis Protein-2 is a Possible Target of Novel Treatment for Endometriosis
| Autor: | Fuminori Taniguchi, Hiroko Higaki, Kohkichi Hata, Tasuku Harada, Masao Izawa, Imari Deura, Yukihiro Azuma, Tomio Iwabe, Eriko Hirakawa |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Stromal cell
Immunology Endometriosis Biology Inhibitor of apoptosis Inhibitor of Apoptosis Proteins Endometrium Western blot medicine Humans Immunology and Allergy Molecular Targeted Therapy Phosphorylation Cells Cultured Cell Proliferation medicine.diagnostic_test Tumor Necrosis Factor-alpha Cell growth Tosylphenylalanyl Chloromethyl Ketone Interleukin-8 NF-kappa B Obstetrics and Gynecology Interleukin Molecular biology Gene Expression Regulation Reproductive Medicine Female I-kappa B Proteins Tumor necrosis factor alpha Stromal Cells biological phenomena cell phenomena and immunity Signal transduction Oligopeptides Signal Transduction |
| Zdroj: | American Journal of Reproductive Immunology. 71:278-285 |
| ISSN: | 1046-7408 |
| Popis: | Problem How is the tumor necrosis factor (TNF) α-induced inhibitor of apoptosis (IAP) protein expression in endometriotic stromal cells (ESCs) involved in cell viability and signaling pathways? Method of study Endometriotic stromal cells were isolated from ovarian chocolate cysts in 20 patients who underwent laparoscopic surgery. IAP protein expression and IκB phosphorylation were evaluated by Western blot analysis. Interleukin (IL)-8 protein expression and cell proliferation were assessed by ELISA. Results Cellular IAP (cIAP)-2 protein expression in endometriotic tissue was higher than that of endometrium. TNFα markedly enhanced cIAP-2 protein expression in ESCs. Pretreatment with a nuclear factor (NF)-κB inhibitor attenuated TNFα-induced cIAP-2 expression. An antagonist of IAPs abrogated TNFα-induced cIAP-2 protein expression and showed a decrease in TNFα-induced IL-8 protein expression and BrdU incorporation in ESCs. Conclusions TNFα and its downstream NFκB pathway have proven to be critical regulators of highly expressed cIAP-2 in ESCs. cIAP-2 may be a novel therapeutic target for endometriosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |