Changes in pancreatic histology, insulin secretion and oxidative status in diabetic rats following treatment with Ficus deltoidea and vitexin

Autor: Hafandi Ahmad, Noor Syaffinaz Noor Mohamad Zin, Mahdi Ebrahimi, Sulaiman Md Dom, Samsulrizal Nurdiana, Nur Syimal`ain Azmi, Yong Meng Goh
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Blood Glucose
Male
0301 basic medicine
medicine.medical_treatment
Vitexin
Antioxidants
Rats
Sprague-Dawley
F. deltoidea
chemistry.chemical_compound
Insulin Secretion
Insulin
Medicine
Apigenin
biology
Diabetes
General Medicine
lcsh:Other systems of medicine
Ficus deltoidea
medicine.anatomical_structure
Pancreas
Research Article
medicine.drug
medicine.medical_specialty
Histology
Diabetes Mellitus
Experimental
03 medical and health sciences
Insulin resistance
Internal medicine
Diabetes mellitus
Animals
Humans
Hypoglycemic Agents
Ft-Ir
Plant Extracts
business.industry
Quantitative insulin sensitivity check index
Ficus
biology.organism_classification
Streptozotocin
medicine.disease
lcsh:RZ201-999
Rats
030104 developmental biology
Endocrinology
Complementary and alternative medicine
chemistry
business
Zdroj: BMC Complementary and Alternative Medicine, Vol 17, Iss 1, Pp 1-17 (2017)
BMC Complementary and Alternative Medicine
ISSN: 1472-6882
DOI: 10.1186/s12906-017-1762-8
Popis: The potential application of Ficus deltoidea and vitexin for the management of symptomatologies associated with diabetes mellitus (DM) has gained much attention. However, less firm evidence comes from data to augment our understanding of the role of F. deltoidea and vitexin in protecting pancreatic β-cells. The aim of this study was to assess histological and oxidative stress changes in the pancreas of streptozotocin (STZ)-induced diabetic rats following F. deltoidea extract and vitexin treatment. F. deltoidea and vitexin was administrated orally to six-weeks STZ-induced diabetic rats over 8 weeks period. The glucose and insulin tolerances were assessed by intraperitoneal glucose (2 g/kg) tolerance test (IPGTT) and intraperitoneal insulin (0.65 U/kg) tolerance test (IPITT), respectively. Subsequently, insulin resistance was assessed by homeostasis assessment model of insulin resistance (HOMA-IR), quantitative insulin sensitivity check index (QUICKI) and the insulin/triglyceride-derived McAuley index. The histological changes in the pancreas were then observed by hematoxylin-eosin (H&E) staining. Further, the pattern of fatty acid composition and infrared (IR) spectra of the serum and pancreas were monitored by gas chromatography (GC) method and Fourier Transform Infrared (FT-IR) spectroscopy. F. deltoidea and vitexin increased pancreatic antioxidant enzymes and promoted islet regeneration. However, a significant increase in insulin secretion was observed only in rats treated with F. deltoidea. More importantly, reduction of fasting blood glucose is consistent with reduced FT-IR peaks at 1200-1000 cm−1. These results accentuate that F. deltoidea and vitexin could be a potential agent to attenuate pancreatic oxidative damage and advocate their therapeutic potential for treating DM.
Databáze: OpenAIRE
Externí odkaz: