Characterization of appetite-regulating factors in platyfish, Xiphophorus maculatus (Cyprinodontiformes Poeciliidae)
| Autor: | Paul M. Pitts, Hélène Volkoff |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Cart medicine.medical_specialty Physiology media_common.quotation_subject Appetite Nerve Tissue Proteins Biochemistry Cyprinodontiformes Eating 03 medical and health sciences 0302 clinical medicine Orexigenic Internal medicine mental disorders medicine Animals Neuropeptide Y RNA Messenger 14. Life underwater Molecular Biology Cholecystokinin media_common Orexins biology digestive oral and skin physiology Fasting Feeding Behavior Xiphophorus Neuropeptide Y receptor biology.organism_classification Orexin 030104 developmental biology Endocrinology Gene Expression Regulation nervous system Female Energy Metabolism hormones hormone substitutes and hormone antagonists 030217 neurology & neurosurgery medicine.drug Hormone |
| Zdroj: | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology. 208:80-88 |
| ISSN: | 1095-6433 |
| DOI: | 10.1016/j.cbpa.2017.03.018 |
| Popis: | The regulation of energy in fish, like most vertebrates, is a complex process that involves a number of brain and peripheral hormones. These signals include anorexigenic (e.g. cholecystokinin (CCK) and cocaine- and amphetamine-regulated transcript (CART)) as well as orexigenic (e.g. orexin and neuropeptide Y (NPY)) peptides. Platyfish, Xiphophorus maculatus, are freshwater viviparous fish for which little is known about the endocrine mechanisms regulating feeding. In order to elucidate the role of these peptides in the regulation of feeding of platyfish, we examined the effects of peripheral injections of CCK and orexin on feeding behavior and food intake. Injections of CCK decreased both food intake and searching behavior, while injections of orexin increased searching behavior but did not affect food consumption. In order to better characterize these peptides, we examined their mRNA tissue distribution and assessed the effects of a 10-day fast on their brain and intestine expressions in both males and females. CCK, CART, NPY and orexin all show widespread distributions in brain and several peripheral tissues, including intestine and gonads. Fasting induced decreases in both CCK and CART and an increase in orexin mRNA expressions in the brain and a decrease in CCK expression in the intestine, but did not affect either expressions of NPY. There were no significant sex-specific differences in either the behavioral responses to injections or the expression responses to fasting. The widespread distribution and the fasting-induced changes in expression of these peptides suggest that they might have several physiological roles in platyfish, including the regulation of feeding. |
| Databáze: | OpenAIRE |
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