Assessment of cellular and molecular changes in the rat brain after gamma radiation and radioprotection by anisomycin

Autor: Marko Dakovic, Biljana Božić Nedeljković, Ilinka Pećinar, Danica Bajuk-Bogdanović, Dušica M Kočović, Pavle R. Andjus
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Premedication
Health
Toxicology and Mutagenesis
Radiation-Protective Agents
Pharmacology
anisomycin
Raman microspectroscopy
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Nucleic Acids
Blood plasma
Animals
Radiology
Nuclear Medicine and imaging
Irradiation
Fundamental Radiation Science
Cobalt Radioisotopes
Rats
Wistar
Interleukin 6
Anisomycin
030304 developmental biology
0303 health sciences
Radiation
biology
Chemistry
radioprotectors
Calcium-Binding Proteins
Microfilament Proteins
gamma radiation
Brain
Rat brain
cytokines
Rats
3. Good health
glial cells
Radiation Injuries
Experimental
Interleukin 10
Gamma Rays
Astrocytes
030220 oncology & carcinogenesis
Nucleic acid
biology.protein
Immunohistochemistry
AcademicSubjects/SCI00960
Microglia
AcademicSubjects/MED00870
Cranial Irradiation
Corrigendum
Zdroj: Journal of Radiation Research
ISSN: 1349-9157
0449-3060
Popis: The objective of the study was to describe cellular and molecular markers of radioprotection by anisomycin, focusing on the changes in rat brain tissue. Two-month-old Wistar rats were exposed to a 60Co radiation source at a dose of 6 Gy, with or without radioprotection with anisomycin (150 mg/kg) administered subcutaneously 30 min before or 3 or 6 h after irradiation. Survivors were analyzed 30 days after treatment. Astroglial and microglial responses were investigated based on the expression of glial markers assessed with immunohistochemistry, and quantitative changes in brain biomolecules were investigated by Raman microspectroscopy. In addition, blood plasma levels of pro-inflammatory (interleukin 6 and tumor necrosis factor α) and anti-inflammatory (interleukin 10) cytokines were assessed. We found that application of anisomycin either before or after irradiation significantly decreased the expression of the microglial marker Iba-1. We also found an increased intensity of Raman spectral bands related to nucleic acids, as well as an increased level of cytokines when anisomycin was applied after irradiation. This suggests that the radioprotective effects of anisomycin are by decreasing Iba-1 expression and stabilizing genetic material by increasing the level of nucleic acids.
Databáze: OpenAIRE
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