Lipid peroxidation and hepatic antioxidants in alcoholic liver disease
| Autor: | B J Crump, J A Davies, M Davis, D I Thurnham, R D Situnayake, J Gearty |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
medicine.medical_specialty
Alcoholic liver disease Antioxidant medicine.medical_treatment Linoleic acid Antioxidants Lipid peroxidation chemistry.chemical_compound Internal medicine Biopsy medicine Humans Vitamin E Liver Diseases Alcoholic Ethanol medicine.diagnostic_test Spectrum Analysis Gastroenterology Glutathione medicine.disease Endocrinology chemistry Biochemistry Liver Lipid Peroxidation Research Article |
| Zdroj: | Gut. 31(11) |
| ISSN: | 0017-5749 |
| Popis: | The generation of hepatic liver peroxidation by free radicals has been proposed as a mechanism for ethanol induced hepatotoxicity. To investigate this hypothesis, lipid extracts from hepatic needle biopsy specimens from alcoholic subjects were examined for evidence of lipid peroxidation by measuring total conjugated dienes by derivative spectroscopy and, after hydrolysis of hepatic lipid extract and reverse phase high performance liquid chromatography, the molar ratio between a diene-conjugated linoleic acid isomer (18:2 (9,11)) and the parent linoleic acid isomer (18:2(9,12)). Changes were related to hepatic histology, iron deposition, glutathione and vitamin E values. Derivative spectroscopy minima suggestive of diene conjugation were identified at 233 and 242 nm and correlated weakly, suggesting these two minima may represent different classes of lipid dienes. There was a weak relation with inflammatory histological changes in the biopsy specimen but no correlation with hepatic iron grade, glutathione, or vitamin E lipid ratio. The proportion of 18:2(9,11) linoleic acid in hepatic lipids correlated significantly with inflammatory histological features and inversely with hepatic glutathione. Furthermore, hepatic glutathione was lower in biopsy specimens with greater iron staining. The ratio of vitamin E to lipid was not related to histological group, inflammation, or iron grade. These findings suggest that excess alcohol consumption leads to hepatic inflammation and lipid peroxidation. |
| Databáze: | OpenAIRE |
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