The planocerid flatworm is a main supplier of toxin to tetrodotoxin-bearing fish juveniles
| Autor: | Misato Abe, Kaede Noguchi, Rei Suo, Ayano Kishiki, Tomohiro Takatani, Osamu Arakawa, Haruo Sugita, Takenori Wada, Hikaru Oyama, Maho Kashitani, Tatsunori Sato, Taiki Okabe, Shun Teranishi, Mitsuki Takei, Tomoko Koito, Shiro Itoi, Riko Yamada, Hiroyuki Akagi, Ryuya Ogata |
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| Rok vydání: | 2019 |
| Předmět: |
Environmental Engineering
Food Chain Health Toxicology and Mutagenesis 0208 environmental biotechnology Zoology 02 engineering and technology Tetrodotoxin 010501 environmental sciences medicine.disease_cause 01 natural sciences Food chain chemistry.chemical_compound medicine Environmental Chemistry Juvenile Animals heterocyclic compounds Phylogeny 0105 earth and related environmental sciences Flatworm Larva biology Toxin Tetraodontiformes musculoskeletal neural and ocular physiology fungi Public Health Environmental and Occupational Health Goby General Medicine General Chemistry biology.organism_classification Pollution 020801 environmental engineering Perciformes nervous system chemistry Platyhelminths Marine toxin |
| Zdroj: | Chemosphere. 249 |
| ISSN: | 1879-1298 |
| Popis: | Tetrodotoxin (TTX), a potent neurotoxin, is found in various phylogenetically diverse taxa. In marine environments, the pufferfish is at the top of the food chain among TTX-bearing organisms. The accumulation of TTX in the body of pufferfish appears to be of the food web that begins with bacteria. It is known that toxic pufferfishes possess TTX from the larval/juvenile stage. However, the source of the TTX is unknown because the maternally sourced TTX is extremely small in quantity. Therefore, the TTX has to be obtained from other organisms or directly from the environment. Here, we report evidence that the source of TTX for toxic fish juveniles including the pufferfish (Chelonodon patoca) and the goby (Yongeichthys criniger) is in the food organisms, as seen in their gut contents. Next generation sequencing analysis for the mitochondrial COI gene showed that the majority of the sequence recovered from intestinal contents of these toxic fishes belonged to the flatworm Planocera multitentaculata, a polyclad flatworm containing highly concentrated TTX from the larval stage. PCR specific to P. multitentaculata also showed that DNA encoding the planocerid COI gene was strongly detected in the intestinal contents of the goby and pufferfish juveniles. Additionally, the planocerid specific COI sequence was detected in the environmental seawater collected from the water around the sampling locations for TTX-bearing fish. These results suggest that planocerid larvae are the major TTX supplier for juveniles of TTX-bearing fish species. |
| Databáze: | OpenAIRE |
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