Oxygen induction of a novel fatty acid n−6 desaturase in the soil protozoon, Acanthamoeba castellanii
| Autor: | Andrew J. Rutter, R. James Henderson, John L. Harwood, David Lloyd, Katie L. Thomas, Derek Herbert |
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| Rok vydání: | 2002 |
| Předmět: |
Fatty Acid Desaturases
chemistry.chemical_classification biology Fatty acid chemistry.chemical_element Substrate (chemistry) Acanthamoeba Cell Biology Biochemistry Oxygen Soil Fatty acid desaturase Enzyme chemistry Enzyme Induction Cytochrome b5 biology.protein Animals Acanthamoeba castellanii NAD+ kinase Molecular Biology Research Article |
| Zdroj: | Biochemical Journal. 368:57-67 |
| ISSN: | 1470-8728 0264-6021 |
| DOI: | 10.1042/bj20020189 |
| Popis: | Induction of fatty acid desaturation is very important for the temperature adaptation of poikilotherms. However, in oxygen-limited late-exponential-phase Acanthamoeba castellanii cultures, oxygen alone was able to induce increased activity of a fatty acid desaturase that converts oleate into linoleate and which has been implicated in the temperature adaptation of this organism. Experiments with Delta(10)-nonadecenoate showed that the enzyme is an n -6 desaturase rather than a Delta(12)-desaturase. It also used preferentially 1-acyl-2-oleoyl-phosphatidylcholine as substrate and NAD(P)H as electron donor. The involvement of cytochrome b (5) as an intermediate electron carrier was shown by difference spectra measurements and anti-(cytochrome b (5)) antibody experiments. Of the three protein components of the desaturase complex, oxygen only increased the activity of the terminal (cyanide-sensitive) protein during n -6 desaturase induction. The induction of this terminal protein paralleled well the increase in overall oleate n -6 desaturation. The ability of oxygen to induce oleate desaturase independently of temperature in this lower eukaryotic animal model is of novel intrinsic interest, as well as being important for the design of future experiments to determine the molecular mechanism of temperature adaptation in poikilotherms. |
| Databáze: | OpenAIRE |
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