Rapid diagnostic test for identifying group B streptococcus
| Autor: | Karen Bishop, Gerald Riddle, Jonathan Faro, Allan R Katz, Sebastian Faro |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Time Factors
medicine.drug_class Antibiotics medicine.disease_cause Group B Microbiology Streptococcus agalactiae Pregnancy Prenatal Diagnosis Streptococcal Infections medicine Humans Pregnancy Complications Infectious Incubation reproductive and urinary physiology Bacteriological Techniques biology business.industry Streptococcus Infant Newborn Obstetrics and Gynecology bacterial infections and mycoses Infectious Disease Transmission Vertical Neonatal infection Polyclonal antibodies Pediatrics Perinatology and Child Health biology.protein bacteria Female Antibody business |
| Zdroj: | American journal of perinatology. 28(10) |
| ISSN: | 1098-8785 |
| Popis: | Neonatal infection with Streptococcus agalactiae (group B streptococcus [GBS]) causes significant morbidity and mortality. A truly rapid diagnostic test for identifying GBS would allow for more timely initiation of antibiotic prophylaxis and also reduce the administration of antibiotics for the prevention of early onset neonatal GBS infection. A stock culture was formed from a laboratory reference strain of GBS and was diluted from 10 (7) to 10 (1) bacteria/mL. Specific concentrations were used to inoculate nitrocellulose membranes (NCMs) that had been coated previously with polyclonal rabbit antibody against GBS. After specific times, the NCMs were removed from the sheep blood agar medium, and horseradish-peroxidase conjugate polyclonal antibody against GBS was added. Bound antibody was detected with diaminobenzidine. After 6 hours of incubation, GBS was detected at concentrations from 10 (7) through 10 (4) bacterial/mL. After 4 hours of incubation, GBS was detected at concentrations from 10 (7) through 10 (5) bacteria/mL. GBS was not detected at 2 hours of incubation. Rapid growth and detection of GBS can be performed, and the results can be reliably attained as early as 4 hours. This is in marked contrast to the 48 to 72 hours required by current methods. |
| Databáze: | OpenAIRE |
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