Experimental Phage Therapy in Treating Klebsiella pneumoniae -Mediated Liver Abscesses and Bacteremia in Mice
| Autor: | Chih-Hsin Hung, Nina Tsao, Chih-Feng Kuo, Chiou-Huey Wang, Ching-Ming Wu |
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| Rok vydání: | 2011 |
| Předmět: |
Necrosis
Phage therapy Klebsiella pneumoniae medicine.medical_treatment Liver Abscess Bacteremia Biology Microbiology Mice medicine Animals Experimental Therapeutics Bacteriophages Pharmacology (medical) Pharmacology Liver injury Liver infection medicine.disease biology.organism_classification Klebsiella Infections Mice Inbred C57BL Infectious Diseases Lytic cycle medicine.symptom Liver abscess |
| Zdroj: | Antimicrobial Agents and Chemotherapy. 55:1358-1365 |
| ISSN: | 1098-6596 0066-4804 |
| DOI: | 10.1128/aac.01123-10 |
| Popis: | Intragastric inoculation of mice with Klebsiella pneumoniae can cause liver abscesses, necrosis of liver tissues, and bacteremia. A newly isolated phage (φNK5) with lytic activity for K. pneumoniae was used to treat K. pneumoniae infection in an intragastric model. Both intraperitoneal and intragastric administration of a single dose of φNK5 lower than 2 × 10 8 PFU at 30 min after K. pneumoniae infection was able to protect mice from death in a dose-dependent manner, but the efficacy achieved with a low dose of φNK5 by intragastric treatment provided the more significant protection. Phage φNK5 administered as late as 24 h after K. pneumoniae inoculation was still protective, while intraperitoneal treatment with phage was more efficient than intragastric treatment as a result of the dissemination of bacteria into the circulation at 24 h postinfection. Surveys of bacterial counts for mice treated with φNK5 by the intraperitoneal route revealed that the bacteria were eliminated effectively from both blood and liver tissue. K. pneumoniae -induced liver injury, such as liver necrosis, as well as blood levels of aspartate aminotransferase and alanine aminotransferase and inflammatory cytokine production, was significantly inhibited by φNK5 treatment. These data suggest that a low dose of φNK5 is a potential therapeutic agent for K. pneumoniae -induced liver infection. |
| Databáze: | OpenAIRE |
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