Poly-ϵ-caprolactone/gel hybrid scaffolds for cartilage tissue engineering
| Autor: | H. W. Chung, Jan C. Schagemann, James S. Fitzsimmons, Shawn W. O'Driscoll, Gregory G. Reinholz, James J. Stone, Eike Mrosek |
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| Rok vydání: | 2009 |
| Předmět: |
Materials science
Polyesters Cell Culture Techniques Biomedical Engineering Type II collagen Biocompatible Materials macromolecular substances engineering.material Chondrocyte Biomaterials Chitosan Glycosaminoglycan chemistry.chemical_compound Chondrocytes Materials Testing medicine Animals Glycosaminoglycans Tissue Engineering Tissue Scaffolds biology technology industry and agriculture Metals and Alloys DNA equipment and supplies musculoskeletal system Molecular biology Cartilage medicine.anatomical_structure chemistry Proteoglycan Polycaprolactone Ceramics and Composites biology.protein engineering Rabbits Biopolymer Gels Type I collagen Biomedical engineering |
| Zdroj: | Journal of Biomedical Materials Research Part A. |
| ISSN: | 1552-4965 1549-3296 |
| DOI: | 10.1002/jbm.a.32521 |
| Popis: | The aim of this study was to determine the suitability of hybrid scaffolds composed of naturally derived biopolymer gels and macroporous poly-ϵ-caprolactone (PCL) scaffolds for neocartilage formation in vitro. Rabbit articular chondrocytes were seeded into PCL/HA (1 wt % hyaluronan), PCL/CS (0.5 wt % chitosan), PCL/F (1:3 fibrin sealant plus aprotinin), and PCL/COL1 (0.24% type I collagen) hybrids and cultured statically for up to 50 days. Growth characteristics were evaluated by histological analysis, scanning electron microscopy, and confocal laser scanning microscopy. Neocartilage was quantified using a dimethyl-methylene blue assay for sulfated glycosaminoglycans (sGAG) and an enzyme-linked immunosorbent assay for type II collagen (COL2), normalized to dsDNA content by fluorescent PicoGreen assay. Chondrocytes were homogenously distributed throughout the entire scaffold and exhibited a predominantly spheroidal shape 1 h after being seeded into scaffolds. Immunofluorescence depicted expanding proteoglycan deposition with time. The sGAG per dsDNA increased in all hybrids between days 25 and 50. PCL/HA scaffolds consistently promoted highest yields. In contrast, total sGAG and total COL2 decreased in all hybrids except PCL/CS, which favored increasing values and a significantly higher total COL2 at day 50. Overall, dsDNA content decreased significantly with time, and particularly between days 3 and 6. The PCL/HA hybrid displayed two proliferation peaks at days 3 and 25, and PCL/COL1 displayed one proliferation peak at day 12. The developed hybrids provided distinct short-term environments for implanted chondrocytes, with not all of them being explicitly beneficial (PCL/F, PCL/COL1). The PCL/HA and PCL/CS hybrids, however, promoted specific neocartilage formation and initial cell retention and are thus promising for cartilage tissue engineering. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res 2010 |
| Databáze: | OpenAIRE |
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