MsrB1 and MICALs Regulate Actin Assembly and Macrophage Function via Reversible Stereoselective Methionine Oxidation
| Autor: | Eranthie Weerapana, Byung Cheon Lee, Dmitri E. Fomenko, Andrei Avanesov, Peter R. Hoffmann, Fu Kun W. Hoffmann, Zalán Péterfi, Richard Moore, Vadim N. Gladyshev, Alaattin Kaya, Yani Zhou, Lionel Tarrago |
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| Přispěvatelé: | Harvard Medical School [Boston] (HMS), American University of Hawaii, Division of Genetics, Department of Medicine, Brigham & Women's Hospital, Harvard Medical School, Biodiversité et Biotechnologie Fongiques (BBF), Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA)-École Centrale de Marseille (ECM), Boston College (BC), University of Nebraska [Lincoln], University of Nebraska System, United States Department of Health & Human Services National Institutes of Health (NIH) - USA AG021518 GM061603 RR017675 RR003061 P20RR016453 AI089999, Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-École Centrale de Marseille (ECM), University of Nebraska–Lincoln |
| Rok vydání: | 2013 |
| Předmět: |
Microtubule-associated protein
[SDV.BC]Life Sciences [q-bio]/Cellular Biology Reductase Article Mixed Function Oxygenases 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Methionine Animals [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Methionine synthase Molecular Biology Actin Cells Cultured 030304 developmental biology chemistry.chemical_classification Mice Knockout 0303 health sciences biology Macrophages Microfilament Proteins Cell Biology Actins Cell biology Oxidative Stress chemistry Biochemistry Methionine Sulfoxide Reductases biology.protein Methionine sulfoxide reductase Selenoprotein Oxidoreductases Microtubule-Associated Proteins Oxidation-Reduction 030217 neurology & neurosurgery Function (biology) |
| Zdroj: | Molecular Cell Molecular Cell, Elsevier, 2013, 51 (3), pp.397-404. ⟨10.1016/j.molcel.2013.06.019⟩ Molecular Cell, 2013, 51 (3), pp.397-404. ⟨10.1016/j.molcel.2013.06.019⟩ |
| ISSN: | 1097-2765 1097-4164 |
| DOI: | 10.1016/j.molcel.2013.06.019 |
| Popis: | Redox control of protein function involves oxidation and reduction of amino acid residues, but the mechanisms and regulators involved are insufficiently understood. Here, we report that in conjunction with Mical proteins, methionine-R-sulfoxide reductase B1 (MsrB1) regulates mammalian actin assembly via stereoselective methionine oxidation and reduction in a reversible, site-specific manner. Two methionine residues in actin are specifically converted to methionine-R-sulfoxide by Mical1 and Mical2 and reduced back to methionine by selenoprotein MsrB1, supporting actin disassembly and assembly, respectively. Macrophages utilize this redox control during cellular activation by stimulating MsrB1 expression and activity as a part of innate immunity. We identified the regulatory role of MsrB1 as a Mical antagonist in orchestrating actin dynamics and macrophage function. More generally, our study shows that proteins can be regulated by reversible site-specific methionine-R-sulfoxidation. |
| Databáze: | OpenAIRE |
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