Distribution and dynamic changes of sphingolipids in blood in response to platelet activation
| Autor: | Felix Dahm, Alicja Bielawska, Antonio Nocito, Jacek Bielawski, K. S. Lang, Jerzy Madon, Panco Georgiev, P.-A. Clavien, Y. A. Hannun, Lars M. Asmis |
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| Rok vydání: | 2006 |
| Předmět: |
Blood Platelets
Platelet Membrane Glycoprotein IIb Spectrometry Mass Electrospray Ionization Ceramide Erythrocytes Cell Separation In Vitro Techniques Ceramides Antibodies Mice chemistry.chemical_compound Species Specificity Sphingosine Leukocytes Animals Humans Platelet Sphingosine-1-phosphate Platelet activation Mice Inbred BALB C Sphingolipids Thrombin Hematology Flow Cytometry Platelet Activation Ceramidase Sphingolipid Cell biology chemistry Biochemistry lipids (amino acids peptides and proteins) Lysophospholipids Signal transduction Chromatography Liquid |
| Zdroj: | Journal of Thrombosis and Haemostasis. 4:2704-2709 |
| ISSN: | 1538-7836 |
| Popis: | Summary. Background: Sphingolipids are signaling molecules in a range of biological processes. While sphingosine-1-phosphate (S1P) is thought to be abundantly stored in platelets and released upon stimulation, knowledge about the distribution and function of other sphingolipids in blood is lacking. Objectives: To analyze the sphingolipid content of blood components with special emphasis on dynamic changes in platelets. Methods: Blood components from mice and humans were prepared by gradient centrifugation and analyzed by liquid chromatography–mass spectrometry. Additionally, murine platelets were activated in vitro and in vivo. Results: Isolated non-activated platelets of mice were devoid of S1P, but instead contained dihydrosphingosine-1-phosphate (dhS1P), along with a high concentration of ceramide. Activation of platelets in vitro led to a loss of dhS1P and an increase in sphingosine, accompanied by a reduction of ceramide content. Platelet activation in vivo led to an immediate and continuous rise of dhS1P in plasma, while S1P remained stable. The sphingolipid distribution of human blood was markedly different from mice. Human platelets contained dhS1P in addition to S1P. Conclusions: Mouse platelets contain dhS1P instead of S1P. Platelet activation causes loss of dhS1P and breakdown of ceramide, implying ceramidase activation. Release of dhS1P from activated platelets might be a novel signaling pathway. Finally, the sphingolipid composition of mouse and human blood shows large differences, which must be considered when studying sphingolipid biology. |
| Databáze: | OpenAIRE |
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