Analysis of spatial lamellar distribution from adaptive-optics second harmonic generation corneal images
| Autor: | Harilaos Ginis, Juan M. Bueno, Mary K. Chessey, Raquel Palacios |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
ocis:(180.4315) Nonlinear microscopy
Materials science business.industry Image Processing Fast Fourier transform Second-harmonic generation Image processing Ellipse eye diseases Atomic and Molecular Physics and Optics symbols.namesake Optics Fourier transform ocis:(170.4470) Ophthalmology symbols Lamellar structure sense organs Spatial frequency ocis:(170.3880) Medical and biological imaging ocis:(110.2960) Image analysis Adaptive optics business Biotechnology |
| Zdroj: | Biomedical Optics Express |
| ISSN: | 2156-7085 |
| DOI: | 10.1364/boe.4.001006 |
| Popis: | The spatial organization of stromal collagen of ex-vivo corneas has been quantified in adaptive-optics second harmonic generation (SHG) images by means of an optimized Fourier transform (FT) based analysis. At a particular depth location, adjacent lamellae often present similar orientations and run parallel to the corneal surface. However this pattern might be combined with interweaved collagen bundles leading to crosshatched structures with different orientations. The procedure here reported provides us with both principal and crosshatched angles. This is also able to automatically distinguish a random distribution from a cross-shaped one, since it uses the ratio of the axes lengths of the best-fitted ellipse of the FT data as an auxiliary parameter. The technique has successfully been applied to SHG images of healthy corneas (both stroma and Bowman’s layer) of different species and to corneas undergoing cross-linking treatment. |
| Databáze: | OpenAIRE |
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