MicroRNA-328-3p facilitates the progression of gastric cancer via KEAP1/NRF2 axis
| Autor: | Ruicheng Yan, Jiasheng Liu, Wen-Xin Dao, Yong-Bin Zheng, Wenhong Deng, Jianfei Luo, Zhe Xiao |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Small interfering RNA NF-E2-Related Factor 2 medicine.disease_cause Biochemistry 03 medical and health sciences Stomach Neoplasms microRNA medicine Humans Viability assay Kelch-Like ECH-Associated Protein 1 030102 biochemistry & molecular biology business.industry Cancer General Medicine medicine.disease KEAP1 MicroRNAs 030104 developmental biology Apoptosis Cancer cell Disease Progression Cancer research business Oxidative stress Signal Transduction |
| Zdroj: | Free Radical Research. 55:595-605 |
| ISSN: | 1029-2470 1071-5762 |
| DOI: | 10.1080/10715762.2021.1923705 |
| Popis: | Gastric cancer is a common lethal malignancy and causes great cancer-related mortality worldwide. MicroRNA (miR)-328-3p is implicated in the progression of various human cancers; however, its role and mechanism in the progression of gastric cancer remain unclear.Human gastric cancer cells were incubated with miR-328-3p mimic, inhibitor or the matched negative control. Cell viability, colony formation, migrative and invasive capacity, cell apoptosis and oxidative stress were measured. To clarify the involvement of nuclear factor-E2-related factor 2 (NRF2) and kelch-like ECH-associated protein 1 (KEAP1), small interfering RNA was used. miR-328-3p was upregulated in human gastric cancer cells and tissues, and its level positively correlated with the progression of gastric cancer. miR-328-3p promoted cell viability, colony formation, migration and invasion, thereby facilitating the progression of gastric cancer. miR-328-3p mimic reduced, while miR-328-3p inhibitor increased apoptosis and oxidative stress of human gastric cancer cells. Mechanistically, miR-328-3p upregulated NRF2 via targeting KEAP1to attenuate excessive free radical production and cell apoptosis. miR-328-3p functions as an oncogenic gene and inhibiting miR-328-3p may help to develop novel therapeutic strategies of human gastric cancer. |
| Databáze: | OpenAIRE |
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