Selective decontamination, induced colonization resistance and connected immunological changes in piglets
| Autor: | L. Mandel, I. Trebichavský, J. Trávníček, M. Talafantová, M. Koukal |
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| Rok vydání: | 1985 |
| Předmět: |
biology
Nalidixic acid Swine animal diseases Spleen Kanamycin General Medicine Colonisation resistance biology.organism_classification Microbiology Leukocyte Count Nystatin medicine.anatomical_structure White blood cell Immunoglobulin A Secretory medicine Animals Germ-Free Life Digestive System Feces Bacteria medicine.drug |
| Zdroj: | Folia Microbiologica. 30:312-318 |
| ISSN: | 1874-9356 0015-5632 |
| Popis: | 14-d-old conventional piglets were picked from normal piggery, washed with disinfectants, placed into isolators suitable for germfree work, fed a sterile diet and treated with peroral antibiotics (nalidixic acid, kanamycin, and nystatin). Beginning with day 5 or 7, Enterobacteriaceae were not found in feces. The absence of these bacteria was proved by inoculation of germfree newborn piglets with caecal content. In selectively decontaminated piglets, the white blood cell count in blood had fallen to 6 X 10(9)/L; this decrease was due to an extremely low number of granulocytes (to 0.8 X 10(9)/L). On day 35, IgG-positive cells almost disappeared from the spleen, whereas IgA cells were found in an unusually great amount. Corresponding changes in serum levels were established. The colonization resistance effect in Enterobacteriaceae-deprived piglets was confirmed; settling of introduced various E. coli strains did not occur or was delayed. |
| Databáze: | OpenAIRE |
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