Production and characterization of a human monoclonal antibody, reactive with a conserved epitope on gp41 of human immunodeficiency virus type I
| Autor: | B. Swerdlow, F. Uytdehaag, J. J. Schalken, R. Van De Akker, Vera J. P. Teeuwsen, K. H. J. Siebelink, Jaap Goudsmit, M. J. Stukart, S. Crush-Stanton, A. D. M. E. Osterhaus |
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| Přispěvatelé: | Other departments |
| Rok vydání: | 1990 |
| Předmět: |
Herpesvirus 4
Human medicine.drug_class viruses Immunology Enzyme-Linked Immunosorbent Assay Biology HIV Antibodies Gp41 Monoclonal antibody Peripheral blood mononuclear cell Binding Competitive Virus Epitope Epitopes Antigen Viral envelope Viral Envelope Proteins hemic and lymphatic diseases Virology medicine Humans chemistry.chemical_classification B-Lymphocytes virus diseases Antibodies Monoclonal Cell Transformation Viral Molecular biology Infectious Diseases chemistry HIV-1 Glycoprotein |
| Zdroj: | AIDS research and human retroviruses, 6(3), 381-392. Mary Ann Liebert Inc. |
| ISSN: | 0889-2229 |
| Popis: | A human Epstein-Barr virus-transformed lymphoblastoid B-cell line was generated from peripheral blood mononuclear cells (PBMC) of an asymptomatic human immunodeficiency virus type I (HIV-1) seropositive donor, which produces a human monoclonal antibody K14 (IgG1), reactive with an epitope on the transmembrane part (gp41) of the envelope glycoprotein of HIV-1. This monoclonal antibody reacts with a lysate of HIV-1-infected H9 cells, gradient purified HIV-1, and a vaccinia recombinant HIV-1 gp160 protein, but not with HIV-2 antigens in an enzyme-linked immunosorbent assay (ELISA). When used as an immobilized ligand in an immune affinity column, K14 selectively purifies gp41 from a HIV-1-infected H9 cell lysate. Although no reactivity was observed in ELISA with a panel of partially overlapping synthetic nonapeptides spanning the whole length of HIV-1 gp41, it was shown to react with recombinant envelope proteins, provided that they did contain amino acids 643-692: deletion of this part resulted in the disappearance of the reactivity. Testing of an extensive panel of the sera from HIV-1 seropositive or seronegative donors from Europe and Africa, including a selected group of donors before and after HIV-1 seroconversion, in a competition ELISA with horseradish peroxidase-conjugated K14, showed that the epitope recognized on gp41 is immunodominant and conserved. K14 does not neutralize HIV-1 infectivity or virus-mediated cell fusion, and does not mediate antibody-dependent cellular cytotoxicity. |
| Databáze: | OpenAIRE |
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