Integrin alpha3beta1 inhibits directional migration and wound re-epithelialization in the skin
Autor: | Norman Sachs, Hans Janssen, Maaike Kreft, Arnoud Sonnenberg, Coert Margadant, Karine Raymond |
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Rok vydání: | 2009 |
Předmět: |
Keratinocytes
Integrin Biology Collagen receptor Mice Cell Movement medicine Animals Keratinocyte migration Hemidesmosome assembly Skin Basement membrane Mice Knockout Wound Healing integumentary system Integrin alpha6beta1 Integrin alpha3beta1 Cell Differentiation Cell Biology Mice Mutant Strains Cell biology medicine.anatomical_structure Immunology biology.protein Epidermis Wound healing Cell Adhesion Molecules |
Zdroj: | Journal of cell science. 122(Pt 2) |
ISSN: | 0021-9533 |
Popis: | Re-epithelialization after skin wounding requires both migration and hyperproliferation of keratinocytes. Laminin-332 is deposited during migration over the provisional matrix. To investigate the function of the laminin-332 binding integrin alpha3beta1 in wound re-epithelialization, we generated Itga3flox/flox; K14-Cre mice lacking the alpha3 subunit specifically in the basal layer of the epidermis. These mice are viable but display several skin defects, including local inflammation, hair loss, basement membrane duplication and microblistering at the dermal-epidermal junction, whereas hemidesmosome assembly and keratinocyte differentiation are not impaired. Wound healing is slightly faster in the absence of integrin alpha3beta1, whereas proliferation, the distribution of other integrins and the deposition of basement membrane proteins in the wound bed are unaltered. In vitro, cell spreading is rescued by increased surface expression of alpha6beta1 integrin in the absence of integrin alpha3. The alpha3-deficient keratinocytes migrate with an increased velocity and persistence, whereas proliferation, growth factor signaling, hemidesmosome assembly, and laminin-332 deposition appeared to be normal. We suggest that integrin alpha3beta1 delays keratinocyte migration during wound re-epithelialization, by binding to the laminin-332 that is newly deposited on the wound bed. |
Databáze: | OpenAIRE |
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