Exploitation of human mesenchymal stromal cell derived matrix towards the structural and functional restoration of the ocular surface
| Autor: | Fei Yu, Chenxi Yan, Dan Yan, Siyi Zhang, Qinke Yao, Chunyi Shao, Liangbo Chen, Hao Sun, Yao Fu, Nianxuan Wu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Stromal cell
Biomedical Engineering Matrix (biology) 03 medical and health sciences 0302 clinical medicine Tissue engineering Animals Humans General Materials Science Amnion 030304 developmental biology 0303 health sciences Decellularization Tissue Engineering biology Chemistry Stem Cells Mesenchymal stem cell Mesenchymal Stem Cells Cell biology Fibronectin Transplantation 030221 ophthalmology & optometry biology.protein Rabbits Stem cell Conjunctiva |
| Zdroj: | Biomaterials Science. 8:4712-4727 |
| ISSN: | 2047-4849 2047-4830 |
| Popis: | Conjunctival restoration is indispensable to help maintain the ocular surface microenvironment by secreting lubricative mucins. However, conventional amniotic membrane transplantation therapy has many limitations in its application due to risks involved with disease transmission and alloreactivity. As decellularized tissues have been frequently used for tissue engineering and adipose mesenchymal stem cells (ADMSCs) have been acknowledged for their low immunogenicity, we fabricated a decellularized matrix of adipose-derived mesenchymal stromal cells (DMA) to study the therapeutic potential of DMA in healing conjunctival defects. In the present study, the fabricated DMA, with certain thickness, exhibited transplantation operability in vivo. When applied in conjunctival defect rabbit models, the sheet of DMA played a substantial role in providing structural support without causing cosmetic difference. Moreover, DMA displayed great superiority in promoting faster wound closure with better stratified epithelium containing more goblet cells than the amniotic membranes (AMs). Mechanistically, compared with the tissue culture plates (TCPs) and TCPs coated with collagen or fibronectin (two of the main components of DMA), DMA exhibited its unique property in maintaining the stem cells of CjECs in an undifferentiated state, which is highly essential for long-term conjunctival reconstruction. In addition, DMA effectively enhanced the proliferation of CjECs by activating stronger phosphorylation of the Akt signaling pathway, the results of which were further verified in the in vivo experiment via the histological examination of p-Akt levels in reconstructed conjunctival epithelium by DMA. Thus, the decellularized matrix of ADMSCs depicts a promising conjunctival substitute in ocular reconstruction. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|