Flow cytometric enumeration of bacterial in the coral surface mucus layer
| Autor: | Kouadio-Ngbesso Nadège, Adingra Antoinette, Yvan Bettarel, Bouvier Thierry, Mai Chi Thanh, Van Ngoc Bui, Got Patrice |
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| Přispěvatelé: | MARine Biodiversity Exploitation and Conservation (UMR MARBEC), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut de Recherche pour le Développement (IRD) |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Coral [SDE.MCG]Environmental Sciences/Global Changes 030106 microbiology Colony Count Microbial Biology Microbiology Fluorescent dyes Flow cytometry 03 medical and health sciences [SDV.EE.ECO]Life Sciences [q-bio]/Ecology environment/Ecosystems Enumeration medicine Animals Molecular Biology Fluorescent Dyes Microscopy Microbial Viability medicine.diagnostic_test Bacteria biology.organism_classification Anthozoa Flow Cytometry Mucus 030104 developmental biology Epibionts Mucus layer Corals [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
| Zdroj: | Journal of Microbiological Methods Journal of Microbiological Methods, Elsevier, 2016, 128, pp.16-19. ⟨10.1016/j.mimet.2016.05.032⟩ |
| ISSN: | 1872-8359 0167-7012 |
| DOI: | 10.1016/j.mimet.2016.05.032⟩ |
| Popis: | International audience; The direct counts of bacteria inhabiting coral mucus were performed by flow cytometry testing four fluorescent dyes (SYBR®Green I, HCS, TOPRO®3, SYTO®62) with three different scleractinian species. Results obtained with SYTO62 were the most reliable based on the comparison with standardized epifluorescence counts and the resolution of cytograms. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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