A novel cDNA from Drosophila encoding a protein with similarity to mammalian cysteine-rich secretory proteins, wasp venom antigen 5, and plant group 1 pathogenesis-related proteins
| Autor: | Gae E. Kovalick, Michael C. Schreiber, J.Colleen Karlo |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Untranslated region
DNA Complementary Protein family Sequence analysis Molecular Sequence Data Wasps Wasp Venoms Biology Complementary DNA Genetics Animals Drosophila Proteins Humans Coding region Amino Acid Sequence RNA Messenger Cloning Molecular Gene Peptide sequence In Situ Hybridization Glycoproteins Plant Proteins Sex Chromosomes Base Sequence Sequence Homology Amino Acid Chromosome Mapping Sequence Analysis DNA General Medicine Blotting Northern Blotting Southern Drosophila melanogaster Insect Proteins Cell Adhesion Molecules Drosophila Protein |
| Zdroj: | Gene. 191:135-141 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/s0378-1119(97)00010-3 |
| Popis: | The CAP protein family is made up of a group of secreted proteins that share sequence similarity. Members of this family are found in animals, plants, and fungi, and their shared sequence similarity suggests that members share a common, but as yet unknown, molecular function. As a first step in defining the function of CAP family proteins, an 878 bp partial cDNA encoding a novel member of the CAP family was cloned by the polymerase chain reaction (PCR) from total RNA of adult Drosophila. The cDNA contained the complete coding sequence for a protein 256 amino acids in length, as well as the complete 3' untranslated region (UTR) and a portion of the 5' UTR. The protein, named Antigen 5-related (Agr), was most similar in sequence to antigen 5 (Ag5), a CAP family member found in social wasps and ants. The corresponding Agr RNA is about 1 kb in length and is present at all stages of development, with highest levels observed in adults. Agr RNA is transcribed from a single gene that is located within region 12F of the X chromosome. The identification of Agr in Drosophila expands the number of known CAP family members to well over four dozen. Further studies of Agr and the gene which encodes this protein using the Drosophila model system may help provide important insight into the molecular functioning of this little known, but increasingly significant protein family. |
| Databáze: | OpenAIRE |
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