Quantitative analysis and virulence phenotypes of atypical enteropathogenic Escherichia coli (EPEC) acquired from diarrheal stool samples from a Midwest US hospital

Autor: Gail Hecht, Maximillian J. Carlino, SA Santiago, AT Harrington, Sarah E. Kralicek, Lalitha Sitaraman
Rok vydání: 2020
Předmět:
Male
0301 basic medicine
Bacterial Adhesion
Enteropathogenic Escherichia coli
0302 clinical medicine
bacterial adherence
Escherichia coli Infections
Escherichia coli Proteins
actin pedestal
Gastroenterology
Middle Aged
Phenotype
Gastroenteritis
Diarrhea
Infectious Diseases
Female
030211 gastroenterology & hepatology
Fimbriae Proteins
medicine.symptom
Research Article
Research Paper
atypical EPEC
Adult
Microbiology (medical)
Virulence Factors
Virulence
Biology
Microbiology
BioFire gastrointestinal panel
Young Adult
03 medical and health sciences
Cell Line
Tumor
parasitic diseases
Multiplex polymerase chain reaction
medicine
Humans
lcsh:RC799-869
Adhesins
Bacterial
bacterial infections and mycoses
Bacterial Load
Atypical epec
030104 developmental biology
Fimbriae
Bacterial
transepithelial electrical resistance
bacteria
lcsh:Diseases of the digestive system. Gastroenterology
Genome
Bacterial
HeLa Cells
Zdroj: Gut Microbes
article-version (VoR) Version of Record
Gut Microbes, Vol 12, Iss 1 (2020)
ISSN: 1949-0984
1949-0976
DOI: 10.1080/19490976.2020.1824562
Popis: Infectious diarrhea causes approximately 179 million illnesses annually in the US. Multiplex PCR assays for enteric pathogens detect enteropathogenic Escherichia coli (EPEC) in 12–29% of diarrheal stool samples from all age groups in developed nations. The aim of this study was to isolate and characterize EPEC from diarrhea samples identified as EPEC positive by BioFire Gastrointestinal Panel (GIP). EPEC is the second most common GIP-detected pathogen, equally present in sole and mixed infections peaking during summer months. EPEC bacterial load is higher in samples with additional pathogens. EPEC-GIP-positive stool samples were cultured on MacConkey II agar and analyzed by colony PCR for eaeA and bfpA to identify and classify EPEC isolates as typical (tEPEC) or atypical (aEPEC). EPEC were not recovered from the majority of stool samples with only 61 isolates obtained from 277 samples; most were aEPEC from adults. bfpA-mRNA was severely diminished in 3 of 4 bfpA-positive isolates. HeLa and SKCO-15 epithelial cells were infected with EPEC isolates and virulence-associated phenotypes, including adherence pattern, attachment level, pedestal formation, and tight junction disruption, were assessed. All aEPEC adherence patterns were represented with diffuse adherence predominating. Attachment rates of isolates adhering with defined adherence patterns were higher than tEPEC lacking bfpA (ΔbfpA). The majority of isolates formpedestals. All but one isolate initially increases but ultimately decreases transepithelial electrical resistance of SKCO-15 monolayers, similar to ΔbfpA. Most isolates severely disrupt occludin; ZO-1 disruption is variable. Most aEPEC isolates induce more robust virulence-phenotypes in vitro than ΔbfpA, but less than tEPEC-E2348/69.
Databáze: OpenAIRE
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